Protein Variants | Comment | Organism |
---|---|---|
N726D | mutant enzyme is resistant to camptothecin, no appreciable decrease in catalytic activity, exhibits a distributive mode of plasmid DNA relaxation compared to a progressive mode of the wild-type enzyme. Activity of the mutant enzyme is optimal at 75-100 mM KCl, compared to 150 mM KCl for the wild-type enzyme | Saccharomyces cerevisiae |
N726S | mutant enzyme is resistant to camptothecin | Saccharomyces cerevisiae |
Y727F | inactive mutant enzyme | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
camptothecin | inhibition of wild-type enzyme, no inhibition of mutant enzymes N726S and N726S | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal activity of wild-type enzyme at 150 mM, maximal activity of mutant enzyme N726D at 75-100 mM | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | wild-type enzyme and mutant enzyme N726H exhibit a distinctively processive mode of plasmid relaxation, mutant enzyme N726D exhibits a distributive mode of plasmid DNA relaxation | Saccharomyces cerevisiae | ? | - |
? |