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Literature summary for 5.6.1.8 extracted from

  • Kameyama, S.; Ichikawa, H.; Sunaga, Y.; Nakata, S.; Saito, Y.; Eiki, T.; Watanabe, S.
    Biochemical characteristics of cardiac myosin: the pH dependence of Ca-ATPase activity, and that of the absorption spectrum of 2,4,6-trinitrophenyl groups attached to myosin (1985), J. Biochem., 97, 625-632.
    View publication on PubMed
Search for more literature for 5.6.1.8

Activating Compound

Activating Compound Comment Organism Structure
actin
-
 Gallus gallus
actin
-
 Sus scrofa
actin
-
 Bos taurus
actin
-
 Oryctolagus cuniculus
dioxane at acidic pH weak stimulation of cardiac myosin ATPase and strong activation of skeletal myosin ATPase Bos taurus
dioxane at acidic pH weak stimulation of cardiac myosin ATPase and strong activation of skeletal myosin ATPase Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzene sulfonate inhibition of cardiac and skeletal myosin Bos taurus
2,4,6-Trinitrobenzene sulfonate inhibition of cardiac and skeletal myosin Gallus gallus
2,4,6-Trinitrobenzene sulfonate inhibition of cardiac and skeletal myosin Oryctolagus cuniculus
2,4,6-Trinitrobenzene sulfonate inhibition of cardiac and skeletal myosin Sus scrofa
acetone strong inhibition of cardiac myosin at alkaline pH, weak inhibition of skeletal myosin at alkaline pH Bos taurus
acetone strong inhibition of cardiac myosin at alkaline pH, weak inhibition of skeletal myosin at alkaline pH Gallus gallus
acetone strong inhibition of cardiac myosin at alkaline pH, weak inhibition of skeletal myosin at alkaline pH Oryctolagus cuniculus
acetone strong inhibition of cardiac myosin at alkaline pH, weak inhibition of skeletal myosin at alkaline pH Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + myosin bound to actin filament at position n Gallus gallus
-
 ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n Sus scrofa
-
 ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n Bos taurus
-
 ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n Oryctolagus cuniculus
-
 ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-
Oryctolagus cuniculus
-
-
-
Sus scrofa
-
 pig
-

Source Tissue

Source Tissue Comment Organism Textmining
cardiac muscle
-
 Sus scrofa
-
cardiac muscle
-
 Bos taurus
-
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + myosin bound to actin filament at position n
-
 Gallus gallus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n
-
 Sus scrofa ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n
-
 Bos taurus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?
ATP + H2O + myosin bound to actin filament at position n
-
 Oryctolagus cuniculus ADP + phosphate + myosin bound to actin filament at position n+1
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 37 cardiac myosin activity increases steadily at acidic pH, at alkaline pH activity increases between 0 and 10°C and declines between 20 and 37°C Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 30 activity of skeletal myosin decreases about 50% after preincubation for 20 min at 30°C and pH 5.7, very slow decrease at pH 8.6, activity of cardiac myosin decreases about 90% after preincubation at 25°C and pH 8.6, very slow decrease at pH 5.7 Gallus gallus
25 30 activity of skeletal myosin decreases about 50% after preincubation for 20 min at 30°C and pH 5.7, very slow decrease at pH 8.6, activity of cardiac myosin decreases about 90% after preincubation at 25°C and pH 8.6, very slow decrease at pH 5.7 Sus scrofa
25 30 activity of skeletal myosin decreases about 50% after preincubation for 20 min at 30°C and pH 5.7, very slow decrease at pH 8.6, activity of cardiac myosin decreases about 90% after preincubation at 25°C and pH 8.6, very slow decrease at pH 5.7 Bos taurus
25 30 activity of skeletal myosin decreases about 50% after preincubation for 20 min at 30°C and pH 5.7, very slow decrease at pH 8.6, activity of cardiac myosin decreases about 90% after preincubation at 25°C and pH 8.6, very slow decrease at pH 5.7 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 cardiac myosin at 25°C Bos taurus
9
-
 cardiac myosin at 15°C, at 15°C maximal activity is lower at acidic pH than at alkaline pH for both cardiac- and skeletal myosin Bos taurus
9
-
 skeletal myosin at 15°C, second (lower) optimum at pH 6, U-shaped curve of pH dependency Oryctolagus cuniculus