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Literature summary for 5.6.1.8 extracted from
- The sequence of the myosin 50-20K loop affects myosin's affinity for actin throughout the actin-myosin ATPase cycle and its maximum ATPase activity (1999), Biochemistry, 38, 3785-3792.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| actin | - |
Gallus gallus |  |
| actin | - |
Oryctolagus cuniculus | |
| actin | - |
Dictyostelium sp. | |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | myosin loop 2 replaced by rabbit skeletal or smooth muscle loop 2 | Dictyostelium sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
ATP | chimeric myosin, Dictyostelium myosin loop 2 replaced by rabbit skeletal myosin loop 2, actin activated | Oryctolagus cuniculus | |
| 0.02 | - |
ATP | chimeric myosin, Dictyostelium myosin loop 2 replaced by rabbit skeletal myosin loop 2, actin activated | Dictyostelium sp. | |
| 0.043 | - |
ATP | subfragment 1 | Dictyostelium sp. | |
| 0.06 | - |
ATP | chimeric myosin, Dictyostelium myosin loop 2 replaced by chicken smooth muscle myosin loop 2, actin activated | Oryctolagus cuniculus | |
| 0.06 | - |
ATP | chimeric myosin, Dictyostelium myosin loop 2 replaced by chicken smooth muscle myosin loop 2, actin activated | Dictyostelium sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + myosin bound to actin filament at position n | Gallus gallus | - |
ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
| ATP + H2O + myosin bound to actin filament at position n | Oryctolagus cuniculus | - |
ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
| ATP + H2O + myosin bound to actin filament at position n | Dictyostelium sp. | - |
ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium sp. | - |
- |
- |
| Gallus gallus | - |
chicken | - |
| Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gizzard smooth muscle | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + myosin bound to actin filament at position n | - |
Gallus gallus | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
| ATP + H2O + myosin bound to actin filament at position n | - |
Oryctolagus cuniculus | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
| ATP + H2O + myosin bound to actin filament at position n | - |
Dictyostelium sp. | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
ATP | basal activity | Dictyostelium sp. | |
| 0.95 | - |
ATP | smooth muscle myosin | Oryctolagus cuniculus | |
| 1.2 | - |
ATP | chimeric myosin, Dictyostelium myosin loop 2 replaced by chicken smooth muscle myosin loop 2, actin activated | Dictyostelium sp. | |
| 3 | - |
ATP | actin activated | Dictyostelium sp. | |
| 3.1 | - |
ATP | myosin subfragment 1, actin activated | Dictyostelium sp. | |
| 5.7 | - |
ATP | chimeric myosin, Dictyostelium myosin loop 2 replaced by rabbit skeletal myosin loop 2, actin activated | Dictyostelium sp. | |
| 20 | - |
ATP | skeletal muscle myosin, actin-activated | Oryctolagus cuniculus | |
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