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Literature summary for 5.6.1.7 extracted from

  • An, Y.J.; Rowland, S.E.; Robb, F.T.; Cha, S.S.
    Purification, crystallization, and preliminary X-ray crystallographic analysis of the group III chaperonin from Carboxydothermus hydrogenoformans (2016), J. Microbiol., 54, 440-444 .
    View publication on PubMed
Search for more literature for 5.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Carboxydothermus hydrogenoformans

Crystallization (Commentary)

Crystallization (Comment) Organism
AMP-PNP-bound enzyme form, vapor batch crystallization method, using 20 mM MgCl2, 100 mM HEPES-NaOH (pH 7.5), and 22% (w/v) polyacrylic acid 5100. ADP-bound enzyme form, vapor batch crystallization method, using 200 mM NH4Cl, 10 mM MgCl2, 50 mM HEPES sodium (pH 7.0), 5% (w/v) polyethylene glycol PEG 8000, and 3% (w/v) 1,8-diaminooctane Carboxydothermus hydrogenoformans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Carboxydothermus hydrogenoformans
-
 ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide Carboxydothermus hydrogenoformans ATCC BAA-161
-
 ADP + phosphate + an unfolded polypeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Carboxydothermus hydrogenoformans Q3AF10
-
-
Carboxydothermus hydrogenoformans ATCC BAA-161 Q3AF10
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, t-Butyl HIC column chromatography, and Superdex 200 gel filtration Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
 Carboxydothermus hydrogenoformans ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide
-
 Carboxydothermus hydrogenoformans ATCC BAA-161 ADP + phosphate + an unfolded polypeptide
-
 ?

Synonyms

Synonyms Comment Organism
CPN
-
 Carboxydothermus hydrogenoformans
group III chaperonin
-
 Carboxydothermus hydrogenoformans

General Information

General Information Comment Organism
metabolism chaperonins are ATP-dependent nanomachines that facilitate protein folding through complex cycles of complex allosteric articulation Carboxydothermus hydrogenoformans