Literature summary for 5.6.1.7 extracted from

Yan, X.; Shi, Q.; Bracher, A.; Milicic, G.; Singh, A.K.; Hartl, F.U.; Hayer-Hartl, M.
GroEL ring separation and exchange in the chaperonin reaction (2018), Cell, 172, 605-617.e11 .

Search for more literature for 5.6.1.7

Protein Variants

Protein Variants	Comment	Organism
A109C	the mutant is defective in ring separation and exchange	Escherichia coli
A109S	the mutant shows mixed-ring formation like the wild type enzyme	Escherichia coli
D155A	the mutant preserves negative inter-ring cooperativity and forms mixed-ring complexes in the presence of cofactor GroES/ATP with an efficiency similar to the wild type enzyme	Escherichia coli
D155A/R197A	the double mutant preserves negative inter-ring cooperativity and forms mixed-ring complexes in the presence of cofactor GroES/ATP with an efficiency similar to the wild type enzyme	Escherichia coli
D398A	the mutant enzyme binds ATP but hydrolyzes it at a very slow rate of less than 2% of the wild type enzyme	Escherichia coli
D87K	the mutant enzyme does not bind nucleotide	Escherichia coli
E461K	the mutant enzyme shows no mixed-ring formation	Escherichia coli
Y203E/G337S/I349E	the mutant enzyme is ATPase active but unable to bind substrate protein and the cofactor GroES	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a folded polypeptide	Escherichia coli	-	ADP + phosphate + an unfolded polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6F5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a folded polypeptide	-	Escherichia coli	ADP + phosphate + an unfolded polypeptide	-	?

Subunits

Subunits	Comment	Organism
tetradecamer	14 * 57000, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
chaperonin	-	Escherichia coli
GroEl	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
GroES	-	Escherichia coli

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Release 2023.1 (January 2023)