Protein Variants | Comment | Organism |
---|---|---|
C138W | at 37°C, the mutant enzyme is indistinguishable in all aspects from the wild type, however, at 25°C, steric hindrances cause the chaperonin to be arrested in a ternary complex form, with both unfolded protein and GroES bound to the same ring of the enzyme. An increase in temperature to more than 30°C is sufficient to restart both target protein refolding and ATPase activity in the mutant enzyme | Escherichia coli |
D398A | ATP-hydrolysis deficient mutant | Escherichia coli |
G192W | the mutant enzyme is capable of binding to GroES in the absence of ATP binding | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetradecamer | 14 * 57000, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
chaperonin | - |
Escherichia coli |
GroEl | - |
Escherichia coli |