Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.1.7 extracted from

  • Illingworth, M.; Salisbury, J.; Li, W.; Lin, D.; Chen, L.
    Effective ATPase activity and moderate chaperonin-cochaperonin interaction are important for the functional single-ring chaperonin system (2015), Biochem. Biophys. Res. Commun., 466, 15-20.
    View publication on PubMed
Search for more literature for 5.6.1.7

Protein Variants

Protein Variants Comment Organism
A399T site-diretected mutagenesis, the mutation weakens the affinity for GroES by about 90fold Escherichia coli
A92T site-diretected mutagenesis, the mutation weakens the affinity for GroES by about 1600fold Escherichia coli
D115N site-diretected mutagenesis, the mutation weakens the affinity for GroES by about 50fold Escherichia coli
E191G site-diretected mutagenesis, the mutation weakens the affinity for GroES by about 300fold Escherichia coli
additional information construction of a single-ring GroELSR. GroELSRA399T-GroES and GroELSRD115N-GroES single-ring systems support cell growth in the same manner as the wild-type double-ring GroELeGroES at 37°C and 42°C, while mutant GroELSRA92T-GroES complements GroEL-GroES at both 37°C and 42°C, and mutant GroELSRE191G-GroES complements GroEL-GroES to a lesser extent at 37°C, but not at 42°C. Activities of functional single-ring GroELSReGroES system mutants, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Escherichia coli
-
 ADP + phosphate + an unfolded polypeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
 Escherichia coli ADP + phosphate + an unfolded polypeptide
-
 ?

Synonyms

Synonyms Comment Organism
GroEl
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
GroES GroES decreases the ATP hydrolysis rate of GroEL by 50% and exerts a greater inhibitory effect on GroELSR than GroEL, as it decreases ATP hydrolysis activity of GroELSR to 5-10% of the intrinsic rate of GroELSR. Interaction analysis between the single-ring GroELSR mutants and GroES, GroES cycles between association with and dissociation off the single-ring GroELSR variants. The diminished ATPase activity of the GroELSReGroES system is due to formation of a highly stable GroELSReGroES complex with a half life t1/2 of about 300 min Escherichia coli