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Literature summary for 5.6.1.7 extracted from

  • Tan, L.; Hu, M.; Yu, S.; Wang, X.; Lu, F.; Liu, F.; Qiu, X.; Song, C.; Sun, Y.; Ding, C.
    Characterization of the chaperonin GroEL in Mycoplasma gallisepticum (2015), Arch. Microbiol., 197, 235-244.
    View publication on PubMed
Search for more literature for 5.6.1.7

Application

Application Comment Organism
medicine the enzyme can be used as a vaccine candidate against Mycoplasma gallisepticum, a common and widespread cause of chronic respiratory disease in poultry Mycoplasma gallisepticum

Cloned(Commentary)

Cloned (Comment) Organism
gene groEL, DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Mycoplasma gallisepticum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mycoplasma gallisepticum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycoplasma gallisepticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 56915.4, mass spectrometry, x * 62000, recombinant His-tagged enzyme, SDS-PAGE Mycoplasma gallisepticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Mycoplasma gallisepticum
-
 ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide Mycoplasma gallisepticum Rlow
-
 ADP + phosphate + an unfolded polypeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Mycoplasma gallisepticum J3T995 gene groEL
-
Mycoplasma gallisepticum Rlow J3T995 gene groEL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycoplasma gallisepticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
 Mycoplasma gallisepticum ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide
-
 Mycoplasma gallisepticum Rlow ADP + phosphate + an unfolded polypeptide
-
 ?
additional information the recombinant enzyme possesses ATPase activity and contributes to the refolding of recombinant MG PrpC protein Mycoplasma gallisepticum ?
-
 ?
additional information the recombinant enzyme possesses ATPase activity and contributes to the refolding of recombinant MG PrpC protein Mycoplasma gallisepticum Rlow ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56915.4, mass spectrometry, x * 62000, recombinant His-tagged enzyme, SDS-PAGE Mycoplasma gallisepticum

Synonyms

Synonyms Comment Organism
chaperonin GroEL
-
 Mycoplasma gallisepticum
GroEl
-
 Mycoplasma gallisepticum
group I chaperonin
-
 Mycoplasma gallisepticum
Hsp60
-
 Mycoplasma gallisepticum
MG GroEL
-
 Mycoplasma gallisepticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Mycoplasma gallisepticum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 activity range, profile overview Mycoplasma gallisepticum

pI Value

Organism Comment pI Value Maximum pI Value
Mycoplasma gallisepticum sequence calculation
-
 5.38

General Information

General Information Comment Organism
evolution GroEL belongs to the heat shock family of proteins (HSPs), also known as Hsp60 or Group I chaperonin Mycoplasma gallisepticum
physiological function the enzyme possesses ATPase activity and contributed to the refolding of MG PrpC protein, it is a protective antigen Mycoplasma gallisepticum