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Literature summary for 5.6.1.7 extracted from

  • Kanzaki, T.; Iizuka, R.; Takahashi, K.; Maki, K.; Masuda, R.; Sahlan, M.; Yebenes, H.; Valpuesta, J.M.; Oka, T.; Furutani, M.; Ishii, N.; Kuwajima, K.; Yohda, M.
    Sequential action of ATP-dependent subunit conformational change and interaction between helical protrusions in the closure of the built-in lid of group II chaperonins (2008), J. Biol. Chem., 283, 34773-34784.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Thermococcus sp.

Protein Variants

Protein Variants Comment Organism
D393A impaired ATP hydrolysis activity Thermococcus sp.
D64A impaired ATP hydrolysis activity Thermococcus sp.
G65C impaired ATP-dependent conformational change ability Thermococcus sp.

Inhibitors

Inhibitors Comment Organism Structure
ATP addititon of ATP impaired the ability of wild-type chaperonin to protect citrate synthase from thermal aggregation Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
-
-
Thermococcus sp. KS-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide folding of green fluorescent protein, assay at 60°C, 10 min Thermococcus sp. ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide folding of green fluorescent protein, assay at 60°C, 10 min Thermococcus sp. KS-1 ADP + phosphate + an unfolded polypeptide
-
 ?

Subunits

Subunits Comment Organism
octamer ATP-dependent subunit allows conformational change of chaperonin Thermococcus sp.

Synonyms

Synonyms Comment Organism
chaperonin
-
 Thermococcus sp.
group II chaperonin
-
 Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at, protein refolding assay Thermococcus sp.
60
-
 assay at, ATPase activity Thermococcus sp.