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Literature summary for 5.6.1.7 extracted from

  • Bross, P.; Naundrup, S.; Hansen, J.; Nielsen, M.N.; Christensen, J.H.; Kruh?ffer, M.; Palmfeldt, J.; Corydon, T.J.; Gregersen, N.; Ang, D.; Georgopoulos, C.; Nielsen, K.L.
    The Hsp60-(p.V98I) mutation associated with Hereditary Spastic Paraplegia SPG13 compromises chaperonin function both in vitro and in vivo (2008), J. Biol. Chem., 283, 15694-15700.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine the Hsp60-p.V98I mutation is associated with Hereditary Spastic Paraplegia Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
into the pET24d vector for expression in Escherichia coli BL21DE3 cells Escherichia coli
into the pET3a vector for expression in Escherichia coli BL21DE3 cells, the pOFX HSP60wt-HSP10wt plasmid is used for establishing a bacterial in vivo system Homo sapiens

Protein Variants

Protein Variants Comment Organism
G67S additional wild-type control, because this variation is present in a Hsp60 cDNA clone Homo sapiens
V98I mutation affects the ATPase activity and results in a dramatically decreased folding activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Escherichia coli 5739
-
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Escherichia coli
-
ADP + phosphate + an unfolded polypeptide
-
ir
ATP + H2O + a folded polypeptide Homo sapiens
-
ADP + phosphate + an unfolded polypeptide
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using cation exchange chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
Escherichia coli ADP + phosphate + an unfolded polypeptide
-
ir
ATP + H2O + a folded polypeptide
-
Homo sapiens ADP + phosphate + an unfolded polypeptide
-
ir

Subunits

Subunits Comment Organism
heptamer heptameric ring Escherichia coli
heptamer heptameric ring Homo sapiens
tetradecamer back-to-back double heptameric ring Escherichia coli
tetradecamer back-to-back double heptameric ring Homo sapiens

Synonyms

Synonyms Comment Organism
chaperonin ATPase
-
Escherichia coli
chaperonin ATPase
-
Homo sapiens
GroEl
-
Escherichia coli
Hsp60
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
ATPase activity assay at room temperature Escherichia coli
22
-
ATPase activity assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
ATPase activity assay Escherichia coli
7.4
-
ATPase activity assay Homo sapiens