Application | Comment | Organism |
---|---|---|
medicine | the Hsp60-p.V98I mutation is associated with Hereditary Spastic Paraplegia | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the pET24d vector for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
into the pET3a vector for expression in Escherichia coli BL21DE3 cells, the pOFX HSP60wt-HSP10wt plasmid is used for establishing a bacterial in vivo system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G67S | additional wild-type control, because this variation is present in a Hsp60 cDNA clone | Homo sapiens |
V98I | mutation affects the ATPase activity and results in a dramatically decreased folding activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Escherichia coli | 5739 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
ir | |
ATP + H2O + a folded polypeptide | Homo sapiens | - |
ADP + phosphate + an unfolded polypeptide | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using cation exchange chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
ir | |
ATP + H2O + a folded polypeptide | - |
Homo sapiens | ADP + phosphate + an unfolded polypeptide | - |
ir |
Subunits | Comment | Organism |
---|---|---|
heptamer | heptameric ring | Escherichia coli |
heptamer | heptameric ring | Homo sapiens |
tetradecamer | back-to-back double heptameric ring | Escherichia coli |
tetradecamer | back-to-back double heptameric ring | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
chaperonin ATPase | - |
Escherichia coli |
chaperonin ATPase | - |
Homo sapiens |
GroEl | - |
Escherichia coli |
Hsp60 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
ATPase activity assay at room temperature | Escherichia coli |
22 | - |
ATPase activity assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
ATPase activity assay | Escherichia coli |
7.4 | - |
ATPase activity assay | Homo sapiens |