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Literature summary for 5.6.1.7 extracted from
- Expression and characterization of the chaperonin molecular machine from the hyperthermophilic archaeon Pyrococcus furiosus (2007), J. Basic Microbiol., 47, 132-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the Pyrococcus furiosus chaperonin gene is amplified by PCR from genomic DNA, cloned into pET21, and expressed in Escherichia coli BL21-Codonplus DE3-RIL cells | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66200 | - |
determined by SDS-PAGE | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Pyrococcus furiosus | - |
ADP + phosphate + an unfolded polypeptide | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8TZL6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the recombinant protein is purified to homogeneity by using ion-exchange and size-exclusion chromatography | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.006 | - |
ATPase activity at 40°C | Pyrococcus furiosus |
| 0.04 | - |
ATPase activity at 98°C | Pyrococcus furiosus |
| 0.053 | - |
the highest ATPase activity is obtained around 88°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Pyrococcus furiosus | ADP + phosphate + an unfolded polypeptide | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PfCPN | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 88 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 98 | - |
Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
ATPase activity assay | Pyrococcus furiosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | predicted pI-value | - |
5 |
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