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Literature summary for 5.6.1.7 extracted from

  • Knapp, S.; Schmidt-Krey, I.; Hebert, H.; Bergman, T.; Jörnvall, H.; Ladenstein, R.
    The molecular chaperonin TF55 from the thermophilic archaeon Sulfolobus solfataricus. A biochemical and structural characterization (1994), J. Mol. Biol., 242, 397-407.
    View publication on PubMed
Search for more literature for 5.6.1.7

Activating Compound

Activating Compound Comment Organism Structure
K+ enhances the phosphorylation of enzyme during hydrolysis Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
electron microscopy image shows a ninefold symmetrical complex about 17.5 nm in height and 16 nm in diameter, with a central cavity of 4.5 nm Saccharolobus solfataricus

General Stability

General Stability Organism
the enzyme complex is resistant to denaturing agents at room temperature and only pH values lower than 2 lead to dissociation Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibits the phosphorylation of enzyme during hydrolysis Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, alpha subunit, plus x * 61000, beta subunit, SDS-PAGE Saccharolobus solfataricus
61000
-
 x * 58000, alpha subunit, plus x * 61000, beta subunit, SDS-PAGE Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
the separated subunits do not reassemble spontaneously but require ATP and Mg2+ for reassociation Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
22°C, stable for several days in presence of up to 8 M urea Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide a phosphorylated intermediate of enzyme exists during hydrolysis Saccharolobus solfataricus ADP + phosphate + an unfolded polypeptide
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, alpha subunit, plus x * 61000, beta subunit, SDS-PAGE Saccharolobus solfataricus
More protein complex of two different but closely related subunits named alpha and beta. The separated subunits require ATP and Mg2+ for reassociation Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 presence of 4 M urea, dissociation Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 dissociation below Saccharolobus solfataricus