Activating Compound | Comment | Organism | Structure |
---|---|---|---|
K+ | enhances the phosphorylation of enzyme during hydrolysis | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
electron microscopy image shows a ninefold symmetrical complex about 17.5 nm in height and 16 nm in diameter, with a central cavity of 4.5 nm | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
the enzyme complex is resistant to denaturing agents at room temperature and only pH values lower than 2 lead to dissociation | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | inhibits the phosphorylation of enzyme during hydrolysis | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, alpha subunit, plus x * 61000, beta subunit, SDS-PAGE | Saccharolobus solfataricus |
61000 | - |
x * 58000, alpha subunit, plus x * 61000, beta subunit, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
the separated subunits do not reassemble spontaneously but require ATP and Mg2+ for reassociation | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
22°C, stable for several days in presence of up to 8 M urea | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | a phosphorylated intermediate of enzyme exists during hydrolysis | Saccharolobus solfataricus | ADP + phosphate + an unfolded polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, alpha subunit, plus x * 61000, beta subunit, SDS-PAGE | Saccharolobus solfataricus |
More | protein complex of two different but closely related subunits named alpha and beta. The separated subunits require ATP and Mg2+ for reassociation | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
presence of 4 M urea, dissociation | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
dissociation below | Saccharolobus solfataricus |