Literature summary for 5.6.1.7 extracted from

Khor, H.K.; Fisher, M.T.; Schoeneich, C.
Potential role of methionine sulfoxide in the inactivation of the chaperone GroEL by hypochlorous acid (HOCl) and peroxynitrite(ONOO-) (2004), J. Biol. Chem., 279, 19486-19493.

General Stability

General Stability	Organism
GroEL is rather insensitive to oxidants produced endogenously during metabolism, such as nitric oxide or hydrogen peroxide, but is efficiently modified and inactivated by reactive species generated by phagocytes, such as peroxynitrite and hypochlorous acid (HOCl). HOCl inactivates through the oxidation of methionine to methionine sulfoxide. In addition to the oxidation of methionine, HOCl causes the conversion of cysteine to cysteic acid and this product may account for the remainder of inactivated GroEL not recoverable through MsrB/A. HOCl produces only negligible yields of 3-chlorotyrosine. The high sensitivity of GroEL toward HOCl and ONOO suggests that this protein may be a target for bacterial killing by phagocytes	Escherichia coli

Organism

GroEL is rather insensitive to oxidants produced endogenously during metabolism, such as nitric oxide or hydrogen peroxide, but is efficiently modified and inactivated by reactive species generated by phagocytes, such as peroxynitrite and hypochlorous acid (HOCl). HOCl inactivates through the oxidation of methionine to methionine sulfoxide. In addition to the oxidation of methionine, HOCl causes the conversion of cysteine to cysteic acid and this product may account for the remainder of inactivated GroEL not recoverable through MsrB/A. HOCl produces only negligible yields of 3-chlorotyrosine. The high sensitivity of GroEL toward HOCl and ONOO suggests that this protein may be a target for bacterial killing by phagocytes

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a folded polypeptide	-	Escherichia coli	ADP + phosphate + an unfolded polypeptide	-	?
additional information	enzyme catalyzes refolding of denatured malate dehydrogenase into the active form	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
GroEl	-	Escherichia coli

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Release 2023.1 (January 2023)