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Literature summary for 5.6.1.7 extracted from

  • Yoshida, T.; Ideno, A.; Suzuki, R.; Yohda, M.; Maruyama, T.
    Two kinds of archaeal group II chaperonin subunits with different thermostability in Thermococcus strain KS-1 (2002), Mol. Microbiol., 44, 761-769.
    View publication on PubMed
Search for more literature for 5.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expression Escherichia coli Thermococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Thermococcus sp.
-
 ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide Thermococcus sp. KS-1
-
 ADP + phosphate + an unfolded polypeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus sp.
-
-
-
Thermococcus sp. KS-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant alpha16mer Thermococcus sp.
recombinant beta16mer Thermococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 alpha16mer, approx. 0.001 mM/min/mg at 90°C, monomer has no ATPase activity Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
 Thermococcus sp. ADP + phosphate + an unfolded polypeptide
-
 ?
ATP + H2O + a folded polypeptide
-
 Thermococcus sp. KS-1 ADP + phosphate + an unfolded polypeptide
-
 ?

Subunits

Subunits Comment Organism
hexadecamer
-
 Thermococcus sp.

Synonyms

Synonyms Comment Organism
chaperonin alpha subunit
-
 Thermococcus sp.
chaperonin beta subunit
-
 Thermococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermococcus sp.
90
-
-
 Thermococcus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 approx. 15% of maximal activity at 40°C Thermococcus sp.
40 90 approx. 40% of maximal activity at 40°C, approx. 10% of maximal activity at 90°C Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 50% loss of activity after 88 min Thermococcus sp.
80
-
 approx. 60% loss of activity after 90 min Thermococcus sp.
80 95 dissociation of alpha16mer into ATPase inactive monomers Thermococcus sp.
80 95 dissociation of beta16mer into ATPase inactive monomers Thermococcus sp.
85
-
 50% loss of activity after 27 min Thermococcus sp.
85
-
 50% loss of activity after 42 min Thermococcus sp.
90
-
 50% loss of activity after 6 min Thermococcus sp.
90
-
 50% loss of activity after 19 min Thermococcus sp.
95
-
 50% loss of activity after 1 min Thermococcus sp.
95
-
 50% loss of activity after 5 min Thermococcus sp.