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Literature summary for 5.6.1.7 extracted from
- Nucleotide-dependent protein folding in the type II chaperonin from the mesophilic archaeon Methanococcus maripaludis (2003), Biochem. J., 371, 669-673.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanococcus maripaludis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 900000 | - |
gel filtration, recombinant Mm-cpn | Methanococcus maripaludis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Methanococcus maripaludis | - |
ADP + phosphate + an unfolded polypeptide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanococcus maripaludis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Mm-cpn, Mono Q, Superdex S-300 | Methanococcus maripaludis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Methanococcus maripaludis | ADP + phosphate + an unfolded polypeptide | - |
? | |
| ATP + H2O + a folded polypeptide | ATP hydrolysis is not essentially required for protein folding, i.e. chaperone activity of Mm-cpn | Methanococcus maripaludis | ADP + phosphate + an unfolded polypeptide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mm-cpn | - |
Methanococcus maripaludis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Methanococcus maripaludis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | approx. 65% of maximal activity at 30°C, approx. 15% of maximal activity at 65°C | Methanococcus maripaludis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
significant loss of activity | Methanococcus maripaludis |
| 70 | - |
complete loss of activity | Methanococcus maripaludis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.99 | - |
ATP | 37°C | Methanococcus maripaludis | |
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Release 2023.1 (January 2023)
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