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Literature summary for 5.6.1.7 extracted from
- Purification and characterization of the chaperonin 10 and chaperonin 60 proteins from Rhodobacter sphaeroides (1991), Biochemistry, 30, 8181-8186.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cpn10 | - |
Cereibacter sphaeroides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli | 5829 | - |
| cytosol | - |
Cereibacter sphaeroides | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
14 * 61000, SDS-PAGE | Cereibacter sphaeroides |
| 889200 | - |
gel filtration | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
? |  |
| ATP + H2O + a folded polypeptide | Cereibacter sphaeroides | - |
ADP + phosphate + an unfolded polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
purple non-sulfur photosynthetic bacterium | - |
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| sucrose density gradient, green A-agarose, Mono Q, Superose-6 | Cereibacter sphaeroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.134 | - |
at 37°C | Cereibacter sphaeroides |
| 0.751 | - |
at 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
? | |
| ATP + H2O + a folded polypeptide | - |
Cereibacter sphaeroides | ADP + phosphate + an unfolded polypeptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetradecamer | 14 * 61000, SDS-PAGE | Cereibacter sphaeroides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
ATP | - |
Cereibacter sphaeroides | |
| 0.75 | - |
ATP | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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