Any feedback?
Literature summary for 5.6.1.7 extracted from
- Purification and characterization of chaperonin 60 and chaperonin 10 from the anaerobic thermophile Thermoanaerobacter brockii (1994), Eur. J. Biochem., 222, 277-284.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cpn10 | - |
Thermoanaerobacter brockii |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop, 5-10 mg/ml protein in 20 mM 4-morpholinepropanesulfonic acid, pH 7, 40 mM MgCl2 and 10% 2-propanol, 15-25% 2-propanol in reservoir, rhomboidal crystals appear overnight | Thermoanaerobacter brockii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Thermoanaerobacter brockii | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57949 | - |
7 * 63000, SDS-PAGE, 7 * 57949, electrospray mass spectrometry | Thermoanaerobacter brockii |
| 63000 | - |
7 * 63000, SDS-PAGE, 7 * 57949, electrospray mass spectrometry | Thermoanaerobacter brockii |
| 450000 | - |
gel filtration | Thermoanaerobacter brockii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Thermoanaerobacter brockii | - |
ADP + phosphate + an unfolded polypeptide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter brockii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| C6 adsorbent, Q Sepharose, 95-98% purity | Thermoanaerobacter brockii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04 | - |
at 50°C | Thermoanaerobacter brockii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Thermoanaerobacter brockii | ADP + phosphate + an unfolded polypeptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heptamer | 7 * 63000, SDS-PAGE, 7 * 57949, electrospray mass spectrometry | Thermoanaerobacter brockii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
ATP | rate per heptamer at 70°C | Thermoanaerobacter brockii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
