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Literature summary for 5.6.1.7 extracted from

  • Cross, S.J.; Cirulea, A.; Poomputsa, K.; Romaniec, M.P.; Freedman, R.B.
    Thermostable chaperonin from Clostridium thermocellum (1996), Biochem. J., 316, 615-622.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Cpn10
-
Acetivibrio thermocellus

Inhibitors

Inhibitors Comment Organism Structure
Cpn10 60% inhibition of authentic Cpn60, 40% inhibition of recombinant Cpn60 Acetivibrio thermocellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Escherichia coli 5829
-
cytosol
-
Acetivibrio thermocellus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
7 * 65000 Acetivibrio thermocellus
450000
-
gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Escherichia coli
-
ADP + phosphate + an unfolded polypeptide
-
?
ATP + H2O + a folded polypeptide Acetivibrio thermocellus
-
ADP + phosphate + an unfolded polypeptide
-
?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
gram-positive, thermophilic anaerobe
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ATP-agarose Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
Escherichia coli ADP + phosphate + an unfolded polypeptide
-
?
ATP + H2O + a folded polypeptide
-
Acetivibrio thermocellus ADP + phosphate + an unfolded polypeptide
-
?

Subunits

Subunits Comment Organism
heptamer 7 * 65000 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 80 authentic and recombinant Cpn60 Acetivibrio thermocellus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
half-life at 70°C, 30 min, less than 5% activity retained after 90 min Escherichia coli
70
-
half-life of 90 min Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
ATP authentic and recombinant enzyme Acetivibrio thermocellus