Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cpn10 | - |
Acetivibrio thermocellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cpn10 | 60% inhibition of authentic Cpn60, 40% inhibition of recombinant Cpn60 | Acetivibrio thermocellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
cytosol | - |
Acetivibrio thermocellus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
7 * 65000 | Acetivibrio thermocellus |
450000 | - |
gel filtration | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | Escherichia coli | - |
ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | Acetivibrio thermocellus | - |
ADP + phosphate + an unfolded polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
gram-positive, thermophilic anaerobe | - |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ATP-agarose | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a folded polypeptide | - |
Escherichia coli | ADP + phosphate + an unfolded polypeptide | - |
? | |
ATP + H2O + a folded polypeptide | - |
Acetivibrio thermocellus | ADP + phosphate + an unfolded polypeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
heptamer | 7 * 65000 | Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | authentic and recombinant Cpn60 | Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
half-life at 70°C, 30 min, less than 5% activity retained after 90 min | Escherichia coli |
70 | - |
half-life of 90 min | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ATP | authentic and recombinant enzyme | Acetivibrio thermocellus |