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Literature summary for 5.6.1.7 extracted from

  • Yoshida, T.; Yohda, M.; Suzuki, M.; Yazaki, K.; Miura, K-I.; Endo, I.
    Characterization of homo-oligomeric complexes of alpha and beta chaperonin subunits from the acidothermophilic archaeon, Sulfolobus sp. strain 7 (1998), Biochem. Biophys. Res. Commun., 242, 640-647.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, alpha and beta subunits Sulfolobus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sulfolobus sp. 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a folded polypeptide Sulfolobus sp.
-
ADP + phosphate + an unfolded polypeptide
-
?
ATP + H2O + a folded polypeptide Sulfolobus sp. 7
-
ADP + phosphate + an unfolded polypeptide
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol homo-oligomeric complexes of recombinant alpha and beta subunits dissociate into monomers in the presence of 15% ethanol Sulfolobus sp.

Organism

Organism UniProt Comment Textmining
Sulfolobus sp.
-
acidothermophilic archaeon
-
Sulfolobus sp. 7
-
acidothermophilic archaeon
-

Purification (Commentary)

Purification (Comment) Organism
70°C, 30 min, hydrophobic chromatography, partially purified alpha and beta subunits Sulfolobus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
at 70°C Sulfolobus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a folded polypeptide
-
Sulfolobus sp. ADP + phosphate + an unfolded polypeptide
-
?
ATP + H2O + a folded polypeptide
-
Sulfolobus sp. 7 ADP + phosphate + an unfolded polypeptide
-
?