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Literature summary for 5.6.1.6 extracted from

  • Mio, K.; Ogura, T.; Mio, M.; Shimizu, H.; Hwang, T.C.; Sato, C.; Sohma, Y.
    Three-dimensional reconstruction of human cystic fibrosis transmembrane conductance regulator chloride channel revealed an ellipsoidal structure with orifices beneath the putative transmembrane domain (2008), J. Biol. Chem., 283, 30300-30310.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged wild-type CFTR in HEK-293 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the CFTR chloride channel is a membrane-integral protein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the mature enzyme is fully glycosylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged wild-type CFTR from HEK-293 cells by anti-FLAG and wheat germ agglutinin affinity chromatographies, and gel filtration Homo sapiens

Subunits

Subunits Comment Organism
More reconstruction of the three-dimensional structure from negatively stained EM images of purified glycosylated mature CFTR, CFTR comprises a small dome-shaped extracellular and membrane-spanning domain and a large cytoplasmic domain with orifices beneath the putative transmembrane domain Homo sapiens

Synonyms

Synonyms Comment Organism
CFTR
-
 Homo sapiens
cystic fibrosis transmembrane conductance regulator
-
 Homo sapiens
More CFTR belongs to an ATP-binding cassette superfamily Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 ion channel activity assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 ion channel activity assay at Homo sapiens