Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | half-maximal activation by 0.05 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Xenopus laevis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation C590V/592V plus mutation of the remaining 16 Cys residues to Ser, or mutation C590L/592L plus mutation of the remaining 16 Cys residues to Ser results in a mutant protein with about 30% of wild-type activity. This Cys-free mutant allows for the sequential introduction of target Cys residues for cross-linking studies. Introduction of S1248C, S549C, S605C, A1374C, A462C, S1347C into the Cys-free mutant and analysis of crosslinks demonstrates that nucleotide-binding domains 1 and 2 interact in a head-to-tail configuration. Protein phosphorylation by protein kinase A promotes formation of the nucleotide-binding domain heterodimer | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P13569 | isoform CFTR | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | nucleotide-binding domains 1 and 2 interact in head-to-tail configuration. Protein phosphorylation by protein kinase A promotes formation of the nucleotide-binding domain heterodimer | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CFTR protein channel requires phosphorylation by PKA before they can be opened by ATP, close upon ATP removal, and are activated half-maximally by 0.05 mM ATP | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | nucleotide-binding domains 1 and 2 interact in head-to-tail configuration. Protein phosphorylation by protein kinase A promotes formation of the nucleotide-binding domain heterodimer | Homo sapiens |