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Literature summary for 5.6.1.5 extracted from

  • Takeuchi, J.; Tamura, T.
    Recombinant ATPases of the yeast 26S proteasome activate protein degradation by the 20S proteasome (2004), FEBS Lett., 565, 39-42.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + polypeptide Saccharomyces cerevisiae
-
ADP + phosphate + unfolded polypeptide
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Rpt subunits Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
artificial Rpt complex consisting of Rpt subunits Rpt4, proteasome ATPase is thought to hydrolyze ATP at the interface between neighboring subunits Saccharomyces cerevisiae
0.88
-
artificial Rpt complex consisting of Rpt subunits Rpt1 and Rpt2, proteasome ATPase is thought to hydrolyze ATP at the interface between neighboring subunits Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + polypeptide
-
Saccharomyces cerevisiae ADP + phosphate + unfolded polypeptide
-
?

Synonyms

Synonyms Comment Organism
Rpt ATPase ATPase complex Saccharomyces cerevisiae