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Literature summary for 5.6.1.4 extracted from

  • Chen, C.J.; Rayment, I.; Gilbert, S.P.
    Kinesin Kar3Cik1 ATPase pathway for microtubule cross-linking (2011), J. Biol. Chem., 286, 29261-29272.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.4

Cloned(Commentary)

Cloned (Comment) Organism
truncated KAR3 (Lys268-Lys729) and truncated CIK1 (Lys252-Asp594) coexpressed in Escherichia coli BL21-CodonPlus(DE3)-RIL yielding a heterodimer Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0271
-
 ATP in presence of microtubles, Vmax/2 at 0.0004 mM microtubles, pH 7.2, 25°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P17119
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Saccharomyces cerevisiae ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

Subunits Comment Organism
heterodimer truncated subunits, predicted 95892 Da (52833 Da + 43059 Da) Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
kinesin Kar3Cik1 ATPase a kinesin-14 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 ATP in presence of microtubles, pH 7.2, 25°C Saccharomyces cerevisiae