Literature summary for 5.6.1.4 extracted from

Heuston, E.; Bronner, C.E.; Kull, F.J.; Endow, S.A.
A kinesin motor in a force-producing conformation (2010), BMC Struct. Biol., 10, 19.

Search for more literature for 5.6.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
residues H293-K700 for crystallization, GST fusion protein (residues K210-K700) for biochemical and motility assays expressed in Escherichia coli BL21(DE3)pLysS or Rosetta2(DE3)pLysS	Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drops,room temperature, PEG 8000	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
T436S	the mutant enzyme permits more rapid nucleotide exchange and moves more slowly along microtubles in gliding assays	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ion exchange chromatography (SP Sepharose) and ion exchange chromatography (MonoQ) or gel filtration	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	-	Drosophila melanogaster	ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)	-	?

Synonyms

Synonyms	Comment	Organism
kinesin-14 Ncd	-	Drosophila melanogaster

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Release 2023.1 (January 2023)