Activating Compound | Comment | Organism | Structure |
---|---|---|---|
microtubule | the solution Klp2 ATPase is activated 15fold more effectively by Schizosaccharomyces pombe microtubules than by pig brain microtubules | Schizosaccharomyces pombe |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
182000 | - |
theoretical molecular weight | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schizosaccharomyces pombe | in microtubule gliding assays using microtubules assembled from pig brain tubulin, Klp2 drives minus-end-directed motility | ? | - |
? | |
additional information | Schizosaccharomyces pombe | Klp2 is required for the proper organization of interphase microtubules into bipolar arrays of parallel-overlapped microtubules | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
6His-tagged full-length Klp2. a Klp2 protein lacking the motor domain (Klp2-tail) and a Klp2 protein containing only the motor domain and a short coiled coil to mediate dimerization (Klp2-motor) are purified by ammonium sulfate precipitation and HisTrapHP column chromatography | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Schizosaccharomyces pombe | ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
additional information | in microtubule gliding assays using microtubules assembled from pig brain tubulin, Klp2 drives minus-end-directed motility | Schizosaccharomyces pombe | ? | - |
? | |
additional information | Klp2 is required for the proper organization of interphase microtubules into bipolar arrays of parallel-overlapped microtubules | Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
kinesin-14 | - |
Schizosaccharomyces pombe |
Klp2 | - |
Schizosaccharomyces pombe |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Schizosaccharomyces pombe | theoretical pI-value | - |
10 |