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Literature summary for 5.6.1.4 extracted from

  • Cai, S.; Weaver, L.N.; Ems-McClung, S.C.; Walczak, C.E.
    Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules (2009), Mol. Biol. Cell, 20, 1348-1359.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HeLa cells Homo sapiens
expressed in Sf9 insect cells Xenopus laevis

Protein Variants

Protein Variants Comment Organism
N593K inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
importin alpha/beta binding of importin alpha/beta to XCTK2 inhibits its association with microtubules and blocks its ability to cross-link microtubules Xenopus laevis
additional information Ran regulates the association of kinesin-14 with importin alpha/beta to prevent aberrant cross-linking and bundling of microtubules by sequestering kinesin-14 in the nucleus during interphase Homo sapiens
additional information XCTK2 is regulated by Ran via the association of a bipartite nuclear localization signal in the tail of XCTK2 with importin alpha/beta, which regulates its ability to cross-link microtubules during spindle formation Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens kinesin-14 acts during mitosis to cross-link and slide between parallel microtubules to regulate spindle length ?
-
 ?
additional information Xenopus laevis kinesin-14 acts during mitosis to cross-link and slide between parallel microtubules to regulate spindle length ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xenopus laevis
glutathione Sepharose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Homo sapiens ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Xenopus laevis ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
additional information kinesin-14 acts during mitosis to cross-link and slide between parallel microtubules to regulate spindle length Homo sapiens ?
-
 ?
additional information kinesin-14 acts during mitosis to cross-link and slide between parallel microtubules to regulate spindle length Xenopus laevis ?
-
 ?

Synonyms

Synonyms Comment Organism
HSET minus-end-directed microtubule motor Homo sapiens
kinesin-14
-
 Homo sapiens
kinesin-14
-
 Xenopus laevis
XCTK2 minus-end-directed microtubule motor Xenopus laevis