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Literature summary for 5.6.1.4 extracted from

  • Furuta, K.; Edamatsu, M.; Maeda, Y.; Toyoshima, Y.Y.
    Diffusion and directed movement: in vitro motile properties of fission yeast kinesin-14 Pkl1 (2008), J. Biol. Chem., 283, 36465-36473.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.4

Activating Compound

Activating Compound Comment Organism Structure
microtubule
-
 Schizosaccharomyces pombe

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) or BL21-CodonPlus (DE3) RIL cells Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
 ATP green fluorescent protein-tagged Pkl1H, in 12 mM PIPES-KOH, 2 mM MgCl2, 1 mM EGTA, pH 6.8, containing 1 mM dithiothreitol, 0.7 mg/ml casein, and 1% (v/v) Tween 20 at 25°C Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schizosaccharomyces pombe single molecules of Pkl1 are not highly processive but only exhibit biased one-dimensional diffusion along microtubules, whereas several molecules of Pkl1, probably fewer than 10 molecules, cooperatively move along microtubules and substantially reduce the diffusive component in the movement ?
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST-bind resin chromatography and NAP-5 gel filtration Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Schizosaccharomyces pombe ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?
additional information single molecules of Pkl1 are not highly processive but only exhibit biased one-dimensional diffusion along microtubules, whereas several molecules of Pkl1, probably fewer than 10 molecules, cooperatively move along microtubules and substantially reduce the diffusive component in the movement Schizosaccharomyces pombe ?
-
 ?

Subunits

Subunits Comment Organism
dimer Pkl1 has a predicted coiled-coil region at amino acid residues 196-481, suggesting that the native form of Pkl1 is at least dimeric Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
kinesin-14
-
 Schizosaccharomyces pombe
Pkl1 Pkl1 is a slow, minus end-directed microtubule motor Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 ATP green fluorescent protein-tagged Pkl1H, in 12 mM PIPES-KOH, 2 mM MgCl2, 1 mM EGTA, pH 6.8, containing 1 mM dithiothreitol, 0.7 mg/ml casein, and 1% (v/v) Tween 20 at 25°C Schizosaccharomyces pombe