Literature summary for 5.6.1.4 extracted from

Robin, G.; DeBonis, S.; Dornier, A.; Cappello, G.; Ebel, C.; Wade, R.H.; Thierry-Mieg, D.; Kozielski, F.
Essential kinesins: characterization of Caenorhabditis elegans KLP-15 (2005), Biochemistry, 44, 6526-6536.

Search for more literature for 5.6.1.4

Activating Compound

Activating Compound	Comment	Organism	Structure
microtubule	ATP hydrolysis is stimulated 100-150fold in the presence of microtubules	Caenorhabditis elegans

Cloned(Commentary)

Cloned (Comment)	Organism
KLP-15 constructs with different length extensions preceding the motor domain are expressed in Escherichia coli	Caenorhabditis elegans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
NaCl	300 mM, stimulates by a factor of up to 2	Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50839	-	2 * 50839, MALDI-TOF MS, the monomer is immobile whereas the fully dimeric KLP-15 construct supports gliding at 0.0023 mm per min and moves towards microtubule minus end	Caenorhabditis elegans
94000	-	gel filtration	Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	Caenorhabditis elegans	dimeric KLP-15 construct supports gliding and moves towards microtubule minus end	ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)	-	?

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	dimeric KLP-15 construct supports gliding and moves towards microtubule minus end	Caenorhabditis elegans	ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)	-	?
ATP + H2O + a kinesin associated with a microtubule at position n	dimeric KLP-15 construct supports gliding at 0.0023 mm per min and moves towards microtubule minus end	Caenorhabditis elegans	ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 50839, MALDI-TOF MS, the monomer is immobile whereas the fully dimeric KLP-15 construct supports gliding at 0.0023 mm per min and moves towards microtubule minus end	Caenorhabditis elegans

Synonyms

Synonyms	Comment	Organism
KLP-15	-	Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	turnover-numbers of KLP-15 constructs with different length extensions preceding the motor domain in presence or absence of 300 mM NaCl and microtubules	Caenorhabditis elegans

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Release 2023.1 (January 2023)