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Literature summary for 5.6.1.3 extracted from
- New insights into the coupling between microtubule depolymerization and ATP hydrolysis by kinesin-13 protein Kif2C (2015), J. Biol. Chem., 290, 18721-18731.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | microtubule-stimulated ATPase | Homo sapiens | |
| tubulin-vinblastine assemblies | stimulation of the ATPase activity of the enzyme | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the wild-type and mutant minimal functional domain of Kif2C, a fragment comprising the proximal part of the neck region and the motor domain | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant minimal functional domain Kif2C-(sN+M)-sL2 in complex with ADP, by vapor diffusion using a crystallization buffer consisting of 0.1 M Tris-HCl, pH 8.0, 1.5 M ammonium sulfate, and 10% ethylene glycol, at 20°C, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement using HsKif2C motor domain-ADP, PDB ID 2HEH, as a starting model | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of L2, DVK, and DSK loop2 mutants of the functional domain of Kif2C, the microtubule depolymerization activity of the DVK mutant is remarkably decreased compared to the wild-type, while the DSK mutant does not show microtubule depolymerization activity even at high concentrations | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000047 | - |
ATP | pH 6.8, 25°C, tubuline-stimulated recombinant Kif2C functional domain mutant DSK | Homo sapiens |  |
| 0.000058 | - |
ATP | pH 6.8, 25°C, tubuline-stimulated recombinant Kif2C functional domain mutant DVK | Homo sapiens | |
| 0.00008 | - |
ATP | pH 6.8, 25°C, tubulin-stimulated recombinant Kif2C functional domain wild-type | Homo sapiens | |
| 0.00018 | - |
ATP | pH 6.8, 25°C, tubulin-stimulated recombinant Kif2C functional domain mutant L2 | Homo sapiens | |
| 0.0006 | - |
ATP | pH 6.8, 25°C, microtubule-stimulated recombinant Kif2C functional domain mutant DSK | Homo sapiens | |
| 0.00062 | - |
ATP | pH 6.8, 25°C, tubulin-vinblastine-stimulated recombinant Kif2C functional domain mutant L2 | Homo sapiens | |
| 0.00105 | - |
ATP | pH 6.8, 25°C, microtubule-stimulated recombinant Kif2C functional domain mutant DVK | Homo sapiens | |
| 0.0013 | - |
ATP | pH 6.8, 25°C, microtubule-stimulated recombinant Kif2C functional domain mutant L2 | Homo sapiens | |
| 0.00388 | - |
ATP | pH 6.8, 25°C, tubulin-vinblastine-stimulated recombinant Kif2C functional domain wild-type | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a kinesin associated with a microtubule at position n | Homo sapiens | - |
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| additional information | Homo sapiens | the KVD motif of kinesin Kif2C interacts directly with tubulin. ATP hydrolysis in Kif2C is not required for tubulin release | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q99661 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a kinesin associated with a microtubule at position n | - |
Homo sapiens | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? | |
| additional information | the KVD motif of kinesin Kif2C interacts directly with tubulin. ATP hydrolysis in Kif2C is not required for tubulin release | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Kif2C | - |
Homo sapiens |
| kinesin-13 protein | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.069 | - |
ATP | pH 6.8, 25°C, tubulin-vinblastine-stimulated recombinant Kif2C functional domain mutant L2 | Homo sapiens | |
| 0.079 | - |
ATP | pH 6.8, 25°C, tubuline-stimulated recombinant Kif2C functional domain mutant DSK | Homo sapiens | |
| 0.1 | - |
ATP | pH 6.8, 25°C, tubuline-stimulated recombinant Kif2C functional domain mutant DVK | Homo sapiens | |
| 0.117 | - |
ATP | pH 6.8, 25°C, tubulin-stimulated recombinant Kif2C functional domain mutant L2 | Homo sapiens | |
| 0.152 | - |
ATP | pH 6.8, 25°C, tubulin-stimulated recombinant Kif2C functional domain wild-type | Homo sapiens | |
| 0.18 | - |
ATP | pH 6.8, 25°C, microtubule-stimulated recombinant Kif2C functional domain mutant DSK | Homo sapiens | |
| 0.21 | - |
ATP | pH 6.8, 25°C, microtubule-stimulated recombinant Kif2C functional domain mutant L2 | Homo sapiens | |
| 0.62 | - |
ATP | pH 6.8, 25°C, tubulin-vinblastine-stimulated recombinant Kif2C functional domain wild-type | Homo sapiens | |
| 0.97 | - |
ATP | pH 6.8, 25°C, microtubule-stimulated recombinant Kif2C functional domain mutant DVK | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the kinesin superfamily | Homo sapiens |
| additional information | kinesin-13s have a clearly longer loop 2 that folds as a beta-hairpin with a conserved KVD motif at its tip. This KVD motif is necessary for microtubule depolymerization by kinesin-13s. Structural modeling of the Kif2C motor domain in its ATP state in complex with tubulin, overview. The beta-hairpin structure of loop 2 is conserved but shortened. In the mutant, as in the wild type protein, the hairpin that displays the KVD motif interacts similarly with the neck helix, but the KVD motif contacts neither the rest of the motor domain nor the neck helix | Homo sapiens |
| physiological function | the interaction of the kinesin-13 Kif2C KVD motif with tubulin is essential. The interaction of the KVD motif quantitatively determines the activities of Kif2C | Homo sapiens |
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