Cloned (Comment) | Organism |
---|---|
His tagged motor domains, amino acid residues 1-368 and 1-358, expressed in Escherichia coli B834(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R234K | motor domain amino acid residues 1-368, overall similar ATP affinity | Homo sapiens |
S233C/R234K | motor domain amino acid residues 1-368, no ATP hydrolyzing mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P52732 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a kinesin associated with a microtubule at position n | - |
Homo sapiens | ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
kinesin-5 (Eg5/KSP) ATPase | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
ADP | motor domain (amino acid residues 1-358), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the absence of microtubles, pH 7.2, temperature not specified in the publication | Homo sapiens | |
0.0033 | - |
ADP | motor domain (amino acid residues 1-368), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the absence of microtubles, pH 7.2, temperature not specified in the publication | Homo sapiens | |
0.048 | - |
ADP | motor domain (amino acid residues 1-368), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the presence of microtubles, pH 7.2, temperature not specified in the publication | Homo sapiens | |
0.054 | - |
ADP | motor domain (amino acid residues 1-358), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the presence of microtubles, pH 7.2, temperature not specified in the publication | Homo sapiens |