Literature summary for 5.6.1.3 extracted from

van den Wildenberg, S.M.; Tao, L.; Kapitein, L.C.; Schmidt, C.F.; Scholey, J.M.; Peterman, E.J.
The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations (2008), Curr. Biol., 18, 1860-1864.

Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 cells	Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
520000	-	full-length enzyme	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Drosophila melanogaster	during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities can facilitate the gradual accumulation of KLP61F within antiparallel interpolar microtubules at the equator, where the motor can generate force to drive poleward flux and pole-pole separation	?	-	?
additional information	Drosophila melanogaster	KLP61F displays a 3fold higher preference for crosslinking microtubules in the antiparallel orientation, this polarity preference is observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity column chromatography and Superose 6 gel filtration	Drosophila melanogaster

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	-	Drosophila melanogaster	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?
additional information	during morphogenesis of the Drosophila embryo mitotic spindle, KLP61F's crosslinking and sliding activities can facilitate the gradual accumulation of KLP61F within antiparallel interpolar microtubules at the equator, where the motor can generate force to drive poleward flux and pole-pole separation	Drosophila melanogaster	?	-	?
additional information	KLP61F displays a 3fold higher preference for crosslinking microtubules in the antiparallel orientation, this polarity preference is observed in the presence of ADP or ATP plus AMPPNP, but not AMPPNP alone	Drosophila melanogaster	?	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Drosophila melanogaster

Synonyms

Synonyms	Comment	Organism
kinesin-5	-	Drosophila melanogaster
KLP61F	-	Drosophila melanogaster

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Release 2023.1 (January 2023)