Literature summary for 5.6.1.3 extracted from

Roehlk, C.; Rohlfs, M.; Leier, S.; Schliwa, M.; Liu, X.; Parsch, J.; Woehlke, G.
Properties of the Kinesin-1 motor DdKif3 from Dictyostelium discoideum (2008), Eur. J. Cell Biol., 87, 237-249.

Search for more literature for 5.6.1.3

Activating Compound

Activating Compound	Comment	Organism	Structure
microtubule	half-maximal activation for full-length protein 0.00029 mM, for truncated form Kif3-592 0.000304 mM, for truncated form Kif3-342 0.002146 mM	Dictyostelium discoideum
potassium acetate	full-length protein and truncated protein Kif3-592, optimum concentration 200 mM leading to maximum speed of 2 micrometer per s	Dictyostelium discoideum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of full-length and truncated Kif3. Kif3-592 consists of amino acids 1-592, comprising motor domain, neck and partial stalk, Kif3-342 consists of amino acids 1-342 and comprises motor domain and partial neck	Dictyostelium discoideum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Dictyostelium discoideum	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35900	-	truncated protein Kif3-342, calculated from Stokes' radius	Dictyostelium discoideum
40000	-	1 * 40000, calculated, truncated protein Kif3-342	Dictyostelium discoideum
68000	-	2 * 131000, calculated, full-length protein. 2 * 68000, calculated, truncated protein Kif3-592	Dictyostelium discoideum
114300	-	truncated protein Kif3-592, calculated from Stokes' radius	Dictyostelium discoideum
131000	-	2 * 131000, calculated, full-length protein. 2 * 68000, calculated, truncated protein Kif3-592	Dictyostelium discoideum
240000	-	full-length protein, calculated from Stokes' radius	Dictyostelium discoideum

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	isoform DdKif3	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	-	Dictyostelium discoideum	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 131000, calculated, full-length protein. 2 * 68000, calculated, truncated protein Kif3-592	Dictyostelium discoideum
monomer	1 * 40000, calculated, truncated protein Kif3-342	Dictyostelium discoideum

Synonyms

Synonyms	Comment	Organism
KIF3	-	Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
45.9	-	ATP	truncated protein Kif3-342, 22°C, pH 6.8	Dictyostelium discoideum
60.5	-	ATP	truncated protein Kif3-592, 22°C, pH 6.8	Dictyostelium discoideum
144	-	ATP	full-length protein, 22°C, pH 6.8	Dictyostelium discoideum

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Release 2023.1 (January 2023)