Literature summary for 5.6.1.3 extracted from

Kikkawa, M.; Sablin, E.P.; Okada, Y.; Yajima, H.; Fletterick, R.J.; Hirokama, N.
Switch-based mechanism of kinesin motors (2004), Nature, 411, 439-445.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of chimaeric KIF1A construct in Escherichia coli	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
KIF1A-ADP crystals are grown by vapour diffusion, 0.002 ml protein solution containing approx. 15 mg/ml protein are mixed with 0.002 ml reservoir buffer consisting of 30% polyethylene glycol 4000, 100 mM Tris-HCl, pH 8.5, 200 mM sodium acetate, 8% sucrose, 4 mM ADP and 10 mM MgCl2, crystals of KIF1A-beta,gamma-methyleneadenosine 5-triphosphate are obtained using 27% polyethylene glycol 4000, 100 mM MES-NaOH, pH 6.5 and 200 mM sodium acetate as reservoir buffer, crystals are soaked in buffer containing 20 mM beta,gamma-methyleneadenosine 5-triphosphate and 40 mM MgCl2 before data collection, crystals of KIF1A and KIF1A-beta,gamma-methyleneadenosine 5-triphosphate diffract to 2.2 and 2.0 A, respectively	Mus musculus

Crystallization (Comment)

Organism

KIF1A-ADP crystals are grown by vapour diffusion, 0.002 ml protein solution containing approx. 15 mg/ml protein are mixed with 0.002 ml reservoir buffer consisting of 30% polyethylene glycol 4000, 100 mM Tris-HCl, pH 8.5, 200 mM sodium acetate, 8% sucrose, 4 mM ADP and 10 mM MgCl2, crystals of KIF1A-beta,gamma-methyleneadenosine 5-triphosphate are obtained using 27% polyethylene glycol 4000, 100 mM MES-NaOH, pH 6.5 and 200 mM sodium acetate as reservoir buffer, crystals are soaked in buffer containing 20 mM beta,gamma-methyleneadenosine 5-triphosphate and 40 mM MgCl2 before data collection, crystals of KIF1A and KIF1A-beta,gamma-methyleneadenosine 5-triphosphate diffract to 2.2 and 2.0 A, respectively

Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	Mus musculus	-	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant KIF1A	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a kinesin associated with a microtubule at position n	-	Mus musculus	ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)	-	?

Synonyms

Synonyms	Comment	Organism
KIF1A	-	Mus musculus