Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.1.2 extracted from

  • Ashizawa, K.; Oyama, N.; Katayama, S.; Narumi, K.; Tatemoto, H.; Tsuzuki, Y.
    Regulation of fowl sperm motility: evidence for the indirect, but not direct, involvement of dynein-ATPase activity on the reversible temperature-dependent immobilization (2013), Theriogenology, 79, 558-565.
    View publication on PubMed
Search for more literature for 5.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
Lys-Phe-Gly-Val-Gly-Phe-Lys
-
 Gallus gallus
additional information stimulation of fowl sperm motility by temperature, Ca2+, and phosphatase inhibition is not simply associated with an increase of flagellar dynein-ATPase activity Gallus gallus
Val-Ala-Ile-Thr-Val-Leu-Val-Lys
-
 Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
EGTA inhibits demembranated sperm motility at 30°C, reversible by Ca2+, is less effective at 40°C Gallus gallus
additional information only intact but also demembranated cytosol-free sperm are motile at 30°C, but inactive at 40°C Gallus gallus
vanadate almost complete inhibition at 0.05 mM and 30-40°C Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at low temperatures, Ca2+ is essential, at the axonemal level, for initiation of the intrinsic motility of fowl spermat 30°C, but this Ca2+-dependent mechanism might be different from that involved in restoration of motility of intact sperm at 40°C Gallus gallus
Mg2+ required Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Gallus gallus
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n Gallus gallus White leghorn
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-
Gallus gallus White leghorn
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
sperm
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Gallus gallus ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n
-
 Gallus gallus White leghorn ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Synonyms

Synonyms Comment Organism
dynein-ATPase
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 higher activity at compared to 30°C Gallus gallus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 only intact but also demembranated cytosol-free sperm are motile at 30°C, but inactive at 40°C Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Gallus gallus

General Information

General Information Comment Organism
additional information potential mechanisms of the reversible temperature-dependent immobilization of fowl sperm Gallus gallus
physiological function indirect, but not direct, involvement of dynein-ATPase activity on the reversible temperature-dependent immobilization, role of the enzyme in sperm motility, overview Gallus gallus