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Literature summary for 5.6.1.2 extracted from

  • Kon, T.; Imamula, K.; Roberts, A.J.; Ohkura, R.; Knight, P.J.; Gibbons, I.R.; Burgess, S.A.; Sutoh, K.
    Helix sliding in the stalk coiled coil of dynein couples ATPase and microtubule binding (2009), Nat. Struct. Mol. Biol., 16, 325-333.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.2

Protein Variants

Protein Variants Comment Organism
L3339C/I3517C 380-kDa motor domain mutant to introduce disulfide cross-linking Dictyostelium discoideum
L3339C/Q3510C 380-kDa motor domain mutant to introduce disulfide cross-linking Dictyostelium discoideum
additional information HFG380: monomeric 380-kDa motor domain (amino acid residue 1383-4725) fused at its N-terminus with a 6xHis-Flag-EGFP tag Dictyostelium discoideum
Q3340C/I3513C 380-kDa motor domain mutant to introduce disulfide cross-linking Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0321
-
 ATP HFG380, microtubule-activated Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Dictyostelium discoideum ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Synonyms

Synonyms Comment Organism
dynein ATPase
-
 Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
 ATP HFG380, basal activity Dictyostelium discoideum
121.1
-
 ATP HFG380, microtubule-activated activity Dictyostelium discoideum