Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.1.2 extracted from

  • Bell, C.W.; Gibbons, I.R.
    Preparation and properties of dynein ATPase (1983), Muscle Nonmuscle Motil., 2, 1-36.
No PubMed abstract available
Search for more literature for 5.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
NaCl 21S dynein 1, latent form, 14fold increase at 1 M NaCl Tripneustes gratilla
Triton X-100
-
 Tripneustes gratilla

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 ATP 21S dynein 1 Tripneustes gratilla
0.11
-
 ATP 12S dynein 2 Tripneustes gratilla

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 alpha1,alpha2,IC1,IC2,IC3,LC1-LC4, 1 * 333000 + 1 * 320000 + 1 * 122000 + 1 * 90000 + 1 * 76000 + 1 * 76000 + 4 * 24000-14000, 21S dynein 1, SDS-PAGE Tripneustes gratilla
90000
-
 alpha1,alpha2,IC1,IC2,IC3,LC1-LC4, 1 * 333000 + 1 * 320000 + 1 * 122000 + 1 * 90000 + 1 * 76000 + 1 * 76000 + 4 * 24000-14000, 21S dynein 1, SDS-PAGE Tripneustes gratilla
122000
-
 alpha1,alpha2,IC1,IC2,IC3,LC1-LC4, 1 * 333000 + 1 * 320000 + 1 * 122000 + 1 * 90000 + 1 * 76000 + 1 * 76000 + 4 * 24000-14000, 21S dynein 1, SDS-PAGE Tripneustes gratilla
320000
-
 alpha1,alpha2,IC1,IC2,IC3,LC1-LC4, 1 * 333000 + 1 * 320000 + 1 * 122000 + 1 * 90000 + 1 * 76000 + 1 * 76000 + 4 * 24000-14000, 21S dynein 1, SDS-PAGE Tripneustes gratilla
333000
-
 alpha1,alpha2,IC1,IC2,IC3,LC1-LC4, 1 * 333000 + 1 * 320000 + 1 * 122000 + 1 * 90000 + 1 * 76000 + 1 * 76000 + 4 * 24000-14000, 21S dynein 1, SDS-PAGE Tripneustes gratilla
720000
-
 12S dynein 2, analytical gel electrophoreses Tripneustes gratilla
1250000
-
 21S dynein 1, analytical centrifugation, sedimentation-diffusion, sedimentation equilibrium Tripneustes gratilla

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Tripneustes gratilla
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n Aequipecten sp.
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n Unio sp.
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n Asterias
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n Colobocentrotus sp.
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Aequipecten sp.
-
 gill
-
Asterias
-
 starfish
-
Colobocentrotus sp.
-
 sea urchin
-
Tripneustes gratilla
-
 sea urchin
-
Unio sp.
-
 gill
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.26
-
 latent activity of 21S dynein 1, increasing activity after exposure to 40°C, Triton X-100 or -SH reagents Tripneustes gratilla
0.8
-
-
 Asterias
2.8
-
-
 Colobocentrotus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Tripneustes gratilla ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n
-
 Aequipecten sp. ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n
-
 Unio sp. ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n
-
 Asterias ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n
-
 Colobocentrotus sp. ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

Subunits Comment Organism
oligomer alpha1,alpha2,IC1,IC2,IC3,LC1-LC4, 1 * 333000 + 1 * 320000 + 1 * 122000 + 1 * 90000 + 1 * 76000 + 1 * 76000 + 4 * 24000-14000, 21S dynein 1, SDS-PAGE Tripneustes gratilla

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 12S dynein 2 Tripneustes gratilla
10.5
-
 21S dynein 1 Tripneustes gratilla

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 gradual increase from pH 6 to pH 9, sharp peak at pH 10.5 Tripneustes gratilla