Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Deschampsia antarctica |
Crystallization (Comment) | Organism |
---|---|
apoenzyme and S189A mutant incomplex with isoliquiritigenin, sitting drop vapor diffusion method, using 0.2Mammonium sulfate, 0.1MMES (pH 7.3), and 29% (w/v) PEG 5000 MME | Deschampsia antarctica |
Protein Variants | Comment | Organism |
---|---|---|
I190M | the mutant shows increased activity compared to the wild type enzyme | Deschampsia antarctica |
K108A | the mutant shows reduced activity compared to the wild type enzyme | Deschampsia antarctica |
M36A | the mutant shows reduced activity compared to the wild type enzyme | Deschampsia antarctica |
M96K | the mutant shows reduced activity compared to the wild type enzyme | Deschampsia antarctica |
Q104E | the mutant shows increased activity compared to the wild type enzyme | Deschampsia antarctica |
S189A | the mutant shows reduced activity compared to the wild type enzyme | Deschampsia antarctica |
S189T | the mutant shows increased activity compared to the wild type enzyme | Deschampsia antarctica |
Y116A | the mutant shows reduced activity compared to the wild type enzyme | Deschampsia antarctica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
naringenin chalcone | mutant enzyme S189A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0048 | - |
naringenin chalcone | mutant enzyme K108A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0048 | - |
naringenin chalcone | mutant enzyme M36A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0053 | - |
isoliquiritigenin | mutant enzyme R34A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0061 | - |
naringenin chalcone | mutant enzyme Q104E, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.007 | - |
naringenin chalcone | mutant enzyme Y116A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.008 | - |
isoliquiritigenin | mutant enzyme I190M, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0085 | - |
naringenin chalcone | wild type enzyme, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0086 | - |
naringenin chalcone | mutant enzyme I190M, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0099 | - |
naringenin chalcone | mutant enzyme S189T, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0105 | - |
naringenin chalcone | mutant enzyme M96K, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0108 | - |
isoliquiritigenin | mutant enzyme S189T, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0112 | - |
isoliquiritigenin | mutant enzyme K108A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0153 | - |
isoliquiritigenin | wild type enzyme, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0164 | - |
isoliquiritigenin | mutant enzyme M36A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0185 | - |
isoliquiritigenin | mutant enzyme S189A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0225 | - |
isoliquiritigenin | mutant enzyme Q104E, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0243 | - |
isoliquiritigenin | mutant enzyme R34M, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.0276 | - |
isoliquiritigenin | mutant enzyme Y116A, at pH 7.6 and 25°C | Deschampsia antarctica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a chalcone | Deschampsia antarctica | - |
a flavanone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deschampsia antarctica | G4U3G8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Deschampsia antarctica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a chalcone | - |
Deschampsia antarctica | a flavanone | - |
? | |
isoliquiritigenin | low activity compared to naringenin chalcone | Deschampsia antarctica | liquiritigenin | - |
? | |
naringenin chalcone | preferred substrate | Deschampsia antarctica | naringenin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chi1 | - |
Deschampsia antarctica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Deschampsia antarctica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
70 | the enzymatic activity rapidly decreases at temperatures over 60°C, and was almost completely lost at 70°C. The enzyme enzyme shows greater than 50% activity after 30 min of preincubation at 40°C, whereas the activity is completely lost after pre-incubation at 60°C | Deschampsia antarctica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
isoliquiritigenin | mutant enzyme R34A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.002 | - |
isoliquiritigenin | mutant enzyme R34M, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.003 | - |
isoliquiritigenin | mutant enzyme K108A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.003 | - |
isoliquiritigenin | mutant enzyme S189A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.005 | - |
isoliquiritigenin | mutant enzyme I190M, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.007 | - |
isoliquiritigenin | mutant enzyme Y116A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.008 | - |
isoliquiritigenin | mutant enzyme M36A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.017 | - |
isoliquiritigenin | mutant enzyme S189T, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.02 | - |
isoliquiritigenin | wild type enzyme, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.023 | - |
isoliquiritigenin | mutant enzyme Q104E, at pH 7.6 and 25°C | Deschampsia antarctica | |
5 | - |
naringenin chalcone | mutant enzyme S189A, at pH 7.6 and 25°C | Deschampsia antarctica | |
7.8 | - |
naringenin chalcone | mutant enzyme M96K, at pH 7.6 and 25°C | Deschampsia antarctica | |
9.6 | - |
naringenin chalcone | mutant enzyme Y116A, at pH 7.6 and 25°C | Deschampsia antarctica | |
35.2 | - |
naringenin chalcone | mutant enzyme M36A, at pH 7.6 and 25°C | Deschampsia antarctica | |
56.9 | - |
naringenin chalcone | mutant enzyme K108A, at pH 7.6 and 25°C | Deschampsia antarctica | |
130.3 | - |
naringenin chalcone | wild type enzyme, at pH 7.6 and 25°C | Deschampsia antarctica | |
134.7 | - |
naringenin chalcone | mutant enzyme Q104E, at pH 7.6 and 25°C | Deschampsia antarctica | |
197.7 | - |
naringenin chalcone | mutant enzyme I190M, at pH 7.6 and 25°C | Deschampsia antarctica | |
228.2 | - |
naringenin chalcone | mutant enzyme S189T, at pH 7.6 and 25°C | Deschampsia antarctica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Deschampsia antarctica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | about 55% activity at pH 7.0, 100% activity at pH around 8.0, about 80% activity at pH 9.0, about 40% activity at pH 10.0 | Deschampsia antarctica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
isoliquiritigenin | mutant enzyme R34M, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.18 | - |
isoliquiritigenin | mutant enzyme S189A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.242 | - |
isoliquiritigenin | mutant enzyme Y116A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.298 | - |
isoliquiritigenin | mutant enzyme K108A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.314 | - |
isoliquiritigenin | mutant enzyme R34A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.508 | - |
isoliquiritigenin | mutant enzyme M36A, at pH 7.6 and 25°C | Deschampsia antarctica | |
0.625 | - |
isoliquiritigenin | mutant enzyme I190M, at pH 7.6 and 25°C | Deschampsia antarctica | |
1.011 | - |
isoliquiritigenin | mutant enzyme Q104E, at pH 7.6 and 25°C | Deschampsia antarctica | |
1.416 | - |
isoliquiritigenin | wild type enzyme, at pH 7.6 and 25°C | Deschampsia antarctica | |
1.543 | - |
isoliquiritigenin | mutant enzyme S189T, at pH 7.6 and 25°C | Deschampsia antarctica | |
743 | - |
naringenin chalcone | mutant enzyme M96K, at pH 7.6 and 25°C | Deschampsia antarctica | |
1380 | - |
naringenin chalcone | mutant enzyme Y116A, at pH 7.6 and 25°C | Deschampsia antarctica | |
2080 | - |
naringenin chalcone | mutant enzyme S189A, at pH 7.6 and 25°C | Deschampsia antarctica | |
7330 | - |
naringenin chalcone | mutant enzyme M36A, at pH 7.6 and 25°C | Deschampsia antarctica | |
11900 | - |
naringenin chalcone | mutant enzyme K108A, at pH 7.6 and 25°C | Deschampsia antarctica | |
15300 | - |
naringenin chalcone | wild type enzyme, at pH 7.6 and 25°C | Deschampsia antarctica | |
22080 | - |
naringenin chalcone | mutant enzyme Q104E, at pH 7.6 and 25°C | Deschampsia antarctica | |
22920 | - |
naringenin chalcone | mutant enzyme I190M, at pH 7.6 and 25°C | Deschampsia antarctica | |
23060 | - |
naringenin chalcone | mutant enzyme S189T, at pH 7.6 and 25°C | Deschampsia antarctica |