Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.5.1.6 extracted from

  • Braune, A.; Engst, W.; Elsinghorst, P.W.; Furtmann, N.; Bajorath, J.; Guetschow, M.; Blaut, M.
    Chalcone isomerase from Eubacterium ramulus catalyzes the ring contraction of flavanonols (2016), J. Bacteriol., 198, 2965-2974 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Eubacterium ramulus

Protein Variants

Protein Variants Comment Organism
H33A inactive Eubacterium ramulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 (+)-taxifolin at pH 6.8 and 23°C Eubacterium ramulus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Eubacterium ramulus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
 gel filtration Eubacterium ramulus

Organism

Organism UniProt Comment Textmining
Eubacterium ramulus V9P0A9
-
-
Eubacterium ramulus DSM 16296 V9P0A9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, Superdex 200 gel filtration, and phenyl Superose gel filtration Eubacterium ramulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-dihydrokaempferol 60% activity compared to (+)-taxifolin Eubacterium ramulus maesopsin
-
 ?
(+)-dihydrokaempferol 60% activity compared to (+)-taxifolin Eubacterium ramulus DSM 16296 maesopsin
-
 ?
(+)-taxifolin
-
 Eubacterium ramulus alphitonin
-
 ?
(+)-taxifolin
-
 Eubacterium ramulus DSM 16296 alphitonin
-
 ?
butein less than 1% activity compared to (+)-taxifolin Eubacterium ramulus (S)-eriodictyol
-
 ?
butein less than 1% activity compared to (+)-taxifolin Eubacterium ramulus DSM 16296 (S)-eriodictyol
-
 ?
additional information no activity with (-)-taxifolin, (-)-dihydrokaempferol, naringenin, or eriodictyol Eubacterium ramulus ?
-
 ?
additional information no activity with (-)-taxifolin, (-)-dihydrokaempferol, naringenin, or eriodictyol Eubacterium ramulus DSM 16296 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Eubacterium ramulus
? x * 32448, calculated from amino acid sequence Eubacterium ramulus

Synonyms

Synonyms Comment Organism
CHI
-
 Eubacterium ramulus
taxifolin-isomerizing enzyme
-
 Eubacterium ramulus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
41
-
-
 Eubacterium ramulus

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8
-
 Eubacterium ramulus