Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Eubacterium ramulus |
Crystallization (Comment) | Organism |
---|---|
in complex with (2S)-naringenin, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate, 0.2 M NaCl, 0.1 M HEPES pH 7.5, 6% (v/v) ethanol, 0.37 mM naringenin chalcone | Eubacterium ramulus |
Protein Variants | Comment | Organism |
---|---|---|
H33A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
H33E | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
H33Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
H73A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
R125A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
Y48F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
General Stability | Organism |
---|---|
the activity is not affected by DMSO concentrations of up to 5% (v/v) | Eubacterium ramulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
naringenin chalcone | mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.0203 | - |
naringenin chalcone | mutant enzyme H33A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.0439 | - |
naringenin chalcone | mutant enzyme H73A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.0567 | - |
naringenin chalcone | mutant enzyme R125A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.0798 | - |
naringenin chalcone | mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.1319 | - |
naringenin chalcone | mutant enzyme H33E, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.354 | - |
naringenin chalcone | wild type enzyme, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
194000 | - |
gel filtration | Eubacterium ramulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-naringenin | Eubacterium ramulus | - |
naringenin chalcone | - |
r | |
naringenin chalcone | Eubacterium ramulus | - |
(2S)-naringenin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium ramulus | V9P0A9 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and Superdex 200 gel filtration | Eubacterium ramulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-naringenin | - |
Eubacterium ramulus | naringenin chalcone | - |
r | |
naringenin chalcone | - |
Eubacterium ramulus | (2S)-naringenin | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 32400, calculated from amino acid sequence | Eubacterium ramulus |
Synonyms | Comment | Organism |
---|---|---|
CHI | - |
Eubacterium ramulus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
naringenin chalcone | mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.2 | - |
naringenin chalcone | mutant enzyme H33E, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
0.6 | - |
naringenin chalcone | mutant enzyme H33A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
784 | - |
naringenin chalcone | mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
1142 | - |
naringenin chalcone | wild type enzyme, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
1313 | - |
naringenin chalcone | mutant enzyme H73A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
1379 | - |
naringenin chalcone | mutant enzyme R125A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
naringenin chalcone | mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
1.6 | - |
naringenin chalcone | mutant enzyme H33E, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
29 | - |
naringenin chalcone | mutant enzyme H33A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
24000 | - |
naringenin chalcone | mutant enzyme R125A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
28000 | - |
naringenin chalcone | mutant enzyme H73A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
32000 | - |
naringenin chalcone | wild type enzyme, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
55000 | - |
naringenin chalcone | mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus |