Literature summary for 5.5.1.6 extracted from

Gall, M.; Thomsen, M.; Peters, C.; Pavlidis, I.V.; Jonczyk, P.; Gruenert, P.P.; Beutel, S.; Scheper, T.; Gross, E.; Backes, M.; Geissler, T.; Ley, J.P.; Hilmer, J.M.; Krammer, G.; Palm, G.J.; Hinrichs, W.; Bornscheuer, U.T.
Enzymatic conversion of flavonoids using bacterial chalcone isomerase and enoate reductase (2014), Angew. Chem. Int. Ed. Engl., 53, 1439-1442.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Eubacterium ramulus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32500	-	SDS-PAGE	Eubacterium ramulus

Organism

Organism	UniProt	Comment	Textmining
Eubacterium ramulus	V9P0A9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
eriodictyol	-	Eubacterium ramulus	eriodictyol chalcone	-	?
homoeriodictyol	-	Eubacterium ramulus	homoeriodictyol chalcone	-	?
naringenin	-	Eubacterium ramulus	naringenin chalcone	-	?
phloretin	-	Eubacterium ramulus	3-(4-hydroxyphenyl)propanoic acid	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 32500, trimers of three dimer units as determined by crystal analysis	Eubacterium ramulus

Synonyms

Synonyms	Comment	Organism
Chalcone isomerase	-	Eubacterium ramulus
CHI	-	Eubacterium ramulus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Eubacterium ramulus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
41	-	recombinant protein maintains 50% of its activity after incubation at 41°C for 6 h	Eubacterium ramulus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.4	7.6	90% activity	Eubacterium ramulus

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Release 2023.1 (January 2023)