Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',4'-Dihydroxy-4-methoxychalcone | substrate inhibition at 0.025 mM and above | Phaseolus vulgaris | |
2',4'-Dimethoxy-4-hydroxychalcone | inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
3,9-dihydroxypterocarpan | inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
4,2',4'-trihydroxychalcone | substrate inhibition at 0.025 mM and above | Phaseolus vulgaris | |
5,7,2',4'-Tetrahydroxy-8-(3,3-dimethylallyl)isoflavanone | i.e. kievitone, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
5,7,2',4'-Tetrahydroxyisoflavanone | i.e. dalbergioidin, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
5,7,2',4'-tetrahydroxyisoflavone | i.e. 2'-hydroxygenistein, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
5,7,4',5'-Tetrahydroxyflavanol | i.e. quercetin, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
5,7,4'-Trihydroxyflavanone | - |
Phaseolus vulgaris | |
5,7,4'-trihydroxyflavonol | - |
Phaseolus vulgaris | |
5,7,4'-trihydroxyisoflavone | i.e. genistein, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
7,12-Dihydroxycoumestan | i.e. coumestrol, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
7-Hydroxy-3',4'-dimethylchromanocoumaran | i.e. phaseollin, inhibits isomerization of 4,2',4'-trihydroxychalcone | Phaseolus vulgaris | |
HgCl2 | - |
Phaseolus vulgaris | |
PCMB | - |
Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
2',4'-Dihydroxy-4-methoxychalcone | - |
Phaseolus vulgaris | |
0.0089 | - |
4,2',4'-trihydroxychalcone | - |
Phaseolus vulgaris | |
0.0133 | - |
4,2',4',6'-Tetrahydroxychalcone | - |
Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 28000, SDS-PAGE | Phaseolus vulgaris |
30000 | - |
gel filtration | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Phaseolus vulgaris | - |
Storage Stability | Organism |
---|---|
-20°C, 5% glycerol, 35% loss of activity after 6 weeks | Phaseolus vulgaris |
4°C, 0.129 mg enzyme per ml, 95% of the original activity is retained after 6 days | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',4'-Dihydroxy-4-methoxychalcone | - |
Phaseolus vulgaris | ? | - |
? | |
4,2',4',6'-Tetrahydroxychalcone | - |
Phaseolus vulgaris | 5,7,4'-Trihydroxyflavanone | - |
? | |
4,2',4'-trihydroxychalcone | i.e. isoliquiritigenin | Phaseolus vulgaris | (2S)-4',7-dihydroxyflavanone | the product is liquiritigenin | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
4,2',4'-trihydroxychalcone | Phaseolus vulgaris |