BRENDA - Enzyme Database
show all sequences of 5.5.1.5

Metabolism of aromatic compounds by fungi. Kinetic properties and mechanism of 3-carboxy-cis,cis-muconate cyclase from Aspergillus niger

Thatcher, D.R.; Cain, R.B.; Eur. J. Biochem. 56, 193-204 (1975)

Data extracted from this reference:

General Stability
General Stability
Organism
rose-bengal-sensitized photooxidation
Aspergillus niger
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Chloro-4-methylmuconolactone
non-competitive
Aspergillus niger
3-chloromuconate
competitive
Aspergillus niger
3-Chloromuconolactone
non-competitive
Aspergillus niger
3-methylmuconate
competitive
Aspergillus niger
adipate
-
Aspergillus niger
AsO2-
-
Aspergillus niger
AsO43-
-
Aspergillus niger
BO33-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
Br-
-
Aspergillus niger
cis-aconitate
-
Aspergillus niger
citraconate
-
Aspergillus niger
citrate
-
Aspergillus niger
Ethoxyformic anhydride
irreversible, presence of the competitive inhibitors cis-aconitate, trans-aconitate and cis,cis-muconate conferrs some protection
Aspergillus niger
F-
-
Aspergillus niger
fumarate
-
Aspergillus niger
glutaconate
-
Aspergillus niger
Glutarate
-
Aspergillus niger
H2PO2-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
I-
-
Aspergillus niger
Itaconate
-
Aspergillus niger
malate
-
Aspergillus niger
Maleate
-
Aspergillus niger
malonate
-
Aspergillus niger
mesaconate
-
Aspergillus niger
NO2-
-
Aspergillus niger
NO3-
-
Aspergillus niger
oxalate
-
Aspergillus niger
phosphate
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
pimelate
-
Aspergillus niger
S2O32-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
SO42-
-
Aspergillus niger
succinate
-
Aspergillus niger
Tartrate
-
Aspergillus niger
trans,trans-muconate
-
Aspergillus niger
trans-aconitate
-
Aspergillus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
3-carboxy-cis,cis-muconate
-
Aspergillus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-Carboxy-cis,cis-muconate
-
3718
Aspergillus niger
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Aspergillus niger
General Stability (protein specific)
General Stability
Organism
rose-bengal-sensitized photooxidation
Aspergillus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Chloro-4-methylmuconolactone
non-competitive
Aspergillus niger
3-chloromuconate
competitive
Aspergillus niger
3-Chloromuconolactone
non-competitive
Aspergillus niger
3-methylmuconate
competitive
Aspergillus niger
adipate
-
Aspergillus niger
AsO2-
-
Aspergillus niger
AsO43-
-
Aspergillus niger
BO33-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
Br-
-
Aspergillus niger
cis-aconitate
-
Aspergillus niger
citraconate
-
Aspergillus niger
citrate
-
Aspergillus niger
Ethoxyformic anhydride
irreversible, presence of the competitive inhibitors cis-aconitate, trans-aconitate and cis,cis-muconate conferrs some protection
Aspergillus niger
F-
-
Aspergillus niger
fumarate
-
Aspergillus niger
glutaconate
-
Aspergillus niger
Glutarate
-
Aspergillus niger
H2PO2-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
I-
-
Aspergillus niger
Itaconate
-
Aspergillus niger
malate
-
Aspergillus niger
Maleate
-
Aspergillus niger
malonate
-
Aspergillus niger
mesaconate
-
Aspergillus niger
NO2-
-
Aspergillus niger
NO3-
-
Aspergillus niger
oxalate
-
Aspergillus niger
phosphate
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
pimelate
-
Aspergillus niger
S2O32-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
Aspergillus niger
SO42-
-
Aspergillus niger
succinate
-
Aspergillus niger
Tartrate
-
Aspergillus niger
trans,trans-muconate
-
Aspergillus niger
trans-aconitate
-
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.057
-
3-carboxy-cis,cis-muconate
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-Carboxy-cis,cis-muconate
-
3718
Aspergillus niger
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Aspergillus niger
Other publictions for EC 5.5.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730426
Michielse
Degradation of aromatic compou ...
Fusarium oxysporum
Mol. Plant Pathol.
13
1089-1100
2012
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-
-
-
-
-
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
3720
Mazur
3-Carboxy-cis-cis-muconate lac ...
Aspergillus niger, Neurospora crassa
J. Bacteriol.
176
1718-1728
1994
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-
1
-
-
-
-
3
-
1
3
-
-
2
-
1
1
1
-
-
2
-
3
1
-
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
1
3
-
-
-
1
1
-
-
2
-
3
1
-
-
-
3
1
-
-
-
-
-
-
-
-
-
3719
Cook
-
Enzymes of the 3-oxoadipate pa ...
Amorphotheca resinae, Aspergillus amstelodami, Aspergillus fischeri, Aspergillus flavus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Aspergillus terreus, Byssochlamys spectabilis, Clonostachys rosea, Kernia nitida, Monascus ruber, Penicilliopsis clavariiformis, Penicillium capsulatum, Penicillium commune, Penicillium terrestre, Trichoderma deliquescens
J. Gen. Microbiol.
102
81-94
1977
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-
-
-
-
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-
-
-
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-
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18
-
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-
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-
-
18
-
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-
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-
-
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-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3717
Huber
Aromatic metabolism in the fun ...
Rhodotorula mucilaginosa
Arch. Microbiol.
102
139-144
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
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-
1
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-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
3718
Thatcher
Metabolism of aromatic compoun ...
Aspergillus niger
Eur. J. Biochem.
56
193-204
1975
-
-
-
-
-
1
35
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
35
-
1
-
-
-
-
-
-
-
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-
-
-
-
1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
3714
Thatcher
Metabolism of aromatic compoun ...
Aspergillus niger
Eur. J. Biochem.
48
549-556
1974
-
-
-
-
-
-
1
-
-
-
2
1
-
4
-
-
1
-
-
1
1
1
2
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
1
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-
-
-
2
1
-
-
-
1
-
1
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3715
Thatcher
Metabolism of aromatic compoun ...
Aspergillus niger
Eur. J. Biochem.
48
557-562
1974
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-
-
-
-
-
-
-
-
-
1
-
-
4
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
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-
1
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-
1
-
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3716
Cook
Regulation of aromatic metabol ...
Rhodotorula mucilaginosa
J. Gen. Microbiol.
85
37-50
1974
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-
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-
2
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1
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3
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2
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3
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