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Literature summary for 5.5.1.4 extracted from

  • Huang, X.; Hernick, M.
    Recombinant expression of a functional myo-inositol-1-phosphate synthase (MIPS) in Mycobacterium smegmatis (2015), Protein J., 34, 380-390 .
    View publication on PubMed
Search for more literature for 5.5.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells and Mycobacterium smegmatis strain mc24517 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Glucose 6-phosphate Mycobacterium tuberculosis
-
 L-myo-Inositol 1-phosphate
-
 ?
D-Glucose 6-phosphate Mycobacterium tuberculosis H37Rv
-
 L-myo-Inositol 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
IMAC column chromatography and DEAE column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glucose 6-phosphate
-
 Mycobacterium tuberculosis L-myo-Inositol 1-phosphate
-
 ?
D-Glucose 6-phosphate
-
 Mycobacterium tuberculosis H37Rv L-myo-Inositol 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 41000, Halo-tagged protein, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
MIPS
-
 Mycobacterium tuberculosis
Myo-inositol-1-phosphate synthase
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NAD+ required Mycobacterium tuberculosis