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Literature summary for 5.5.1.20 extracted from

  • Kasahara, K.; Miyamoto, T.; Fujimoto, T.; Oguri, H.; Tokiwano, T.; Oikawa, H.; Ebizuka, Y.; Fujii, I.
    Solanapyrone synthase, a possible Diels-Alderase and iterative type I polyketide synthase encoded in a biosynthetic gene cluster from Alternaria solani (2010), ChemBioChem, 11, 1245-1252.
    View publication on PubMed
Search for more literature for 5.5.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae under the starch inducible alpha-amylase promoter, expression in Pichia pastoris Alternaria solani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prosolanapyrone III Alternaria solani the enzyme is involved in solanapyrone biosynthesis (-)-solanapyrone A
-
 ?
prosolanapyrone III Alternaria solani 548 the enzyme is involved in solanapyrone biosynthesis (-)-solanapyrone A
-
 ?

Organism

Organism UniProt Comment Textmining
Alternaria solani D7UQ40
-
-
Alternaria solani 548 D7UQ40
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alternaria solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prosolanapyrone III the enzyme is involved in solanapyrone biosynthesis Alternaria solani (-)-solanapyrone A
-
 ?
prosolanapyrone III the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A. Prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 Alternaria solani (-)-solanapyrone A
-
 ?
prosolanapyrone III the enzyme is involved in solanapyrone biosynthesis Alternaria solani 548 (-)-solanapyrone A
-
 ?
prosolanapyrone III the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A. Prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 Alternaria solani 548 (-)-solanapyrone A
-
 ?

Synonyms

Synonyms Comment Organism
Sol5
-
 Alternaria solani
solanapyrone synthase bifunctional enzyme: prosolanapyrone II oxidase/prosolanapyrone III cycloisomerase Alternaria solani
SPS
-
 Alternaria solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alternaria solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Alternaria solani

Cofactor

Cofactor Comment Organism Structure
flavin flavin-dependent oxidase, the enzyme possesses a flavin-binding domain Alternaria solani