Cloned (Comment) | Organism |
---|---|
expression as His-tagged protein in Escherichia coli | Agrobacterium tumefaciens |
expression as His-tagged protein in Escherichia coli | Hydrogenophaga intermedia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme shows significantly reduced activities in sodium/potassium phosphate/citrate buffers | Agrobacterium tumefaciens | |
additional information | enzyme shows significantly reduced activities in sodium/potassium phosphate/citrate buffers | Hydrogenophaga intermedia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | pH 8.0, 30°C | Agrobacterium tumefaciens | |
0.15 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | pH 8.0, 30°C | Hydrogenophaga intermedia | |
7.1 | - |
3-sulfomuconate | pH 8.0, 30°C | Hydrogenophaga intermedia | |
15.3 | - |
3-sulfomuconate | pH 8.0, 30°C | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Agrobacterium tumefaciens |
42000 | - |
x * 42000, SDS-PAGE | Hydrogenophaga intermedia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens S2 | - |
- |
- |
Hydrogenophaga intermedia | Q2HNY9 | - |
- |
Hydrogenophaga intermedia S1 | Q2HNY9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Agrobacterium tumefaciens |
recombinant protein | Hydrogenophaga intermedia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
pH 8.0, 30°C | Agrobacterium tumefaciens |
0.67 | - |
pH 8.0, 30°C | Hydrogenophaga intermedia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Agrobacterium tumefaciens | cis,cis-butadiene-1,2,4-tricarboxylate | - |
? | |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Agrobacterium tumefaciens S2 | cis,cis-butadiene-1,2,4-tricarboxylate | - |
? | |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Hydrogenophaga intermedia | cis-cis-butadiene-1,2,4-tricarboxylate | - |
? | |
3-sulfomuconate | - |
Agrobacterium tumefaciens | 4-sulfomuconolactone + H2O | - |
? | |
3-sulfomuconate | - |
Hydrogenophaga intermedia | 4-sulfomuconolactone + H2O | - |
? | |
3-sulfomuconate | - |
Agrobacterium tumefaciens S2 | 4-sulfomuconolactone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Agrobacterium tumefaciens |
? | x * 42000, SDS-PAGE | Hydrogenophaga intermedia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | pH 8.0, 30°C | Agrobacterium tumefaciens | |
1.22 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | pH 8.0, 30°C | Hydrogenophaga intermedia | |
20.9 | - |
3-sulfomuconate | pH 8.0, 30°C | Agrobacterium tumefaciens | |
42.7 | - |
3-sulfomuconate | pH 8.0, 30°C | Hydrogenophaga intermedia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Agrobacterium tumefaciens |
8 | - |
- |
Hydrogenophaga intermedia |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
Histidine/HCl buffer, 50% of maximum activity | Agrobacterium tumefaciens |
5.8 | - |
Histidine/HCl buffer, 50% of maximum activity | Hydrogenophaga intermedia |
9 | - |
Tris/HCl buffer, 50% of maximum activity | Agrobacterium tumefaciens |
9 | - |
Tris/HCl buffer, 50% of maximum activity | Hydrogenophaga intermedia |