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Literature summary for 5.5.1.13 extracted from
- Comparison of the enzymatic properties of ent-copalyl diphosphate-synthases in the biosynthesis of phytoalexins and gibberellins in rice (2008), Biosci. Biotechnol. Biochem., 72, 523-530.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-isopropyl-N,N,N,2-tetramethyl-4-[(1-piperidinylcarbonyl)oxy]anilinium chloride | CPS1 activity exhibits approximately 70% inhibition by 0.1 mM Amo-1618, whereas the OsCPS2/OsCyc2 activity exhibits approximately 10% inhibition | Oryza sativa |  |
| Co2+ | 35-60% relative activity in the presence of 0.1 mM Co2+ compared to Mg2+ | Oryza sativa | |
| Fe2+ | 5-10% relative activity in the presence of 0.1 mM Fe+ compared to Mg2+ | Oryza sativa | |
| geranylgeranyl diphosphate | CPS1 but not CPS2/Cyc2 is inhibited by 0.05-0.06 mM geranylgeranyl diphosphate, CPS1 shows significant substrate inhibition in the presence of more than 0.05 mM geranylgeranyl diphosphate | Oryza sativa | |
| Mg2+ | weak inhibition of CPS2/Cyc2 at 5mM Mg2+ | Oryza sativa | |
| Ni2+ | 60-70% relative activity in the presence of 0.1 mM Ni2+ compared to Mg2+ | Oryza sativa | |
| Zn2+ | CPS1 shows 30% enzymatic activity with 0.1 mM Zn2+ compared to Mg2+ | Oryza sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme activity is highest at 0.1mM of Mg2+ | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80900 | - |
CPS2/Cyc2, calculated from amino acid sequence | Oryza sativa |
| 84900 | - |
CPS1, calculated from amino acid sequence | Oryza sativa |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-mercaptoethanol | 5 mM 2-mercaptoethanol is required for full enzymatic activity | Oryza sativa |
| Glycerol | 20% (v/v) glycerol is required for full enzymatic activity | Oryza sativa |
| Tween 80 | 0.1% (v/v) Tween 80 is required for full enzymatic activity | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| copalyl diphosphate | - |
Oryza sativa | ent-kaurene + diphosphate | - |
? | |
| geranylgeranyl diphosphate | - |
Oryza sativa | ent-copalyl diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | gel filtration | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPS1 | isozyme | Oryza sativa |
| CPS2/Cyc2 | bifunctional enzyme, acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins) | Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity is fully retained after incubating at 30°C in 0.05 M Tris-HCl at pH 7.0 for 1 h | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Oryza sativa |
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