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Literature summary for 5.5.1.13 extracted from
- Functional analysis of eubacterial ent-copalyl diphosphate synthase and pimara-9(11),15-diene synthase with unique primary sequences (2007), J. Biochem., 141, 37-45.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 5 mM, required for full activity | Streptomyces sp. |  |
| Tween-80 | 0.1%, required for full activity | Streptomyces sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| geranylgeranyl diphosphate | inhibitory above 0.03 mM | Streptomyces sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0137 | - |
geranylgeranyl diphosphate | 35°C, pH 5.5 | Streptomyces sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | or Zn2+, Mg2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
| Mg2+ | or Zn2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
| Zn2+ | or Mg2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. | - |
- |
- |
| Streptomyces sp. KO-3988 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate | - |
Streptomyces sp. | ent-copalyl diphosphate | - |
? | |
| geranylgeranyl diphosphate | - |
Streptomyces sp. KO-3988 | ent-copalyl diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Streptomyces sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
pH 5.5, stable for at least 1 h | Streptomyces sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
geranylgeranyl diphosphate | 35°C, pH 5.5 | Streptomyces sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Streptomyces sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | more than 90% of maximum activity within this range | Streptomyces sp. |
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