Cloned (Comment) | Organism |
---|---|
expression as myc-fusion protein using Spodoptera frugiperda 21 insect cells. Sequence contains a DVDD motif responsible for copalyl diphosphate synthase activity and a DDYFD motif responsible for ent-kaurene synthase activity | Physcomitrium patens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | sequence contains a DVDD motif responsible for copalyl diphosphate synthase activity and a DDYFD motif responsible for ent-kaurene synthase activity. Mutation of DVDD motif to AVAD leads to loss of function, mutation of DDYFD motif to AAYFD results in accumulation of ent-copalyl diphosphate | Physcomitrium patens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
x * 101000, calculated, x * 106000, SDs-PAGE of myc-fusion protein | Physcomitrium patens |
106000 | - |
x * 101000, calculated, x * 106000, SDs-PAGE of myc-fusion protein | Physcomitrium patens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physcomitrium patens | - |
bifunctional copalyl diphosphate synthase/ent-kaurene synthase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | bifunctional copalyl diphosphate synthase/ent-kaurene synthase | Physcomitrium patens | ent-16-alpha-hydroxy-kaurene + ent-kaurene + diphosphate | 86% and 14%, resp. | ? | |
geranylgeranyl diphosphate | - |
Physcomitrium patens | copalyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 101000, calculated, x * 106000, SDs-PAGE of myc-fusion protein | Physcomitrium patens |