Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli of a recombinant GST fused enzyme | Cucurbita maxima |
expression in Escherichia coli of a recombinant GST fused enzyme | Phaeosphaeria sp. |
Protein Variants | Comment | Organism |
---|---|---|
D320A | the mutation leads to complete enzyme activity | Phaeosphaeria sp. |
D656A | the mutation causes a small reduction in activity | Phaeosphaeria sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibits activity in cell free extract containing the enzyme | Phaeosphaeria sp. | |
additional information | substrate inhibition | Cucurbita maxima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00023 | - |
copalyl diphosphate | at pH 8 | Phaeosphaeria sp. | |
0.00873 | - |
geranylgeranyl diphosphate | at pH 8 | Phaeosphaeria sp. | |
0.0169 | - |
geranylgeranyl diphosphate | at pH 8 | Cucurbita maxima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
calculated from amino acid sequence | Phaeosphaeria sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita maxima | - |
pumpkin | - |
Phaeosphaeria sp. | O13284 | from mycelia | - |
Purification (Comment) | Organism |
---|---|
cell free extract | Phaeosphaeria sp. |
recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis | Phaeosphaeria sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00075 | - |
at pH 8 | Phaeosphaeria sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
copalyl diphosphate | bifunctional enzyme | Phaeosphaeria sp. | ent-kaurene + diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Cucurbita maxima | copalyl diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Phaeosphaeria sp. | copalyl diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00117 | - |
copalyl diphosphate | at pH 8 | Phaeosphaeria sp. | |
0.0308 | - |
geranylgeranyl diphosphate | at pH 8 | Phaeosphaeria sp. | |
0.035 | - |
geranylgeranyl diphosphate | at pH 8 | Cucurbita maxima |