Literature summary for 5.5.1.13 extracted from

Toyomasu, T.; Kawaide, H.; Ishizaki, A.; Shinoda, S.; Otsuka, M.; Mitsuhashi, W.; Sassa, T.
Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase (2000), Biosci. Biotechnol. Biochem., 64, 660-664.

Search for more literature for 5.5.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of a recombinant GST fused enzyme	Fusarium fujikuroi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
107000	-	calculated from amino acid sequence	Fusarium fujikuroi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
copalyl diphosphate	Fusarium fujikuroi	bifunctional enzyme	ent-kaurene + diphosphate	-	?
geranylgeranyl diphosphate	Fusarium fujikuroi	gibberellin biosynthesis	copalyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Fusarium fujikuroi	Q9UVY5	from strain IFO30336 mycelia	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, using Sepharose 4B glutathione affinity resin	Fusarium fujikuroi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
copalyl diphosphate	bifunctional enzyme	Fusarium fujikuroi	ent-kaurene + diphosphate	-	?
geranylgeranyl diphosphate	-	Fusarium fujikuroi	copalyl diphosphate	-	?
geranylgeranyl diphosphate	gibberellin biosynthesis	Fusarium fujikuroi	copalyl diphosphate	-	?

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Release 2023.1 (January 2023)